Molecular genetic characterization of the RD-114 gene family of endogenous feline retroviral sequences.
TLDR
Southern analysis of cat cellular DNA confirmed the conclusions about conserved restriction sites in endogenous sequences and indicated that a single locus may be responsible for the production of the major inducible form of RD-114.Abstract:
RD-114 is a replication-competent, xenotropic retrovirus which is homologous to a family of moderately repetitive DNA sequences present at ca. 20 copies in the normal cellular genome of domestic cats. To examine the extent and character of genomic divergence of the RD-114 gene family as well as to assess their positional association within the cat genome, we have prepared a series of molecular clones of endogenous RD-114 DNA segments from a genomic library of cat cellular DNA. Their restriction endonuclease maps were compared with each other as well as to that of the prototype-inducible RD-114 which was molecularly cloned from a chronically infected human cell line. The endogenous sequences analyzed were similar to each other in that they were colinear with RD-114 proviral DNA, were bounded by long terminal redundancies, and conserved many restriction sites in the gag and pol regions. However, the env regions of many of the sequences examined were substantially deleted. Several of the endogenous RD-114 genomes contained a novel envelope sequence which was unrelated to the env gene of the prototype RD-114 env gene but which, like RD-114 and endogenous feline leukemia virus provirus, was found only in species of the genus Felis, and not in other closely related Felidae genera. The endogenous RD-114 sequences each had a distinct cellular flank which indicates that these sequences are not tandem but dispersed nonspecifically throughout the genome. Southern analysis of cat cellular DNA confirmed the conclusions about conserved restriction sites in endogenous sequences and indicated that a single locus may be responsible for the production of the major inducible form of RD-114.read more
Citations
More filters
Journal ArticleDOI
A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes
TL;DR: A human 293-derived retroviral packaging cell line (293GPG) capable of producing high titers of recombinant Moloney murine leukemia virus particles that have incorporated the vesicular stomatitis virus G (VSV-G) protein is generated.
Journal ArticleDOI
Walker's Mammals of the World, 4th Edition, Ronald M. Nowak, John L. Paradiso. The Johns Hopkins University Press, Baltimore, Maryland (1983), 1xi, +1-568 (Vol. I), xxv+569-1362 (Vol. II). Price $65.00
TL;DR: This book is based on a symposium organized by the Entomological Society of America in 1980 and will prove to be an important book in bringing together recent research on the mating systems of orthopterans, and discussing their behaviour in the light of current theory in behavioura].
Journal ArticleDOI
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
TL;DR: Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant viruses were released by the FLYA13 and FLYRD18 lines.
Journal ArticleDOI
Initial sequence and comparative analysis of the cat genome
Joan Pontius,James C. Mullikin,Douglas Smith,Agencourt Sequencing Team,Kerstin Lindblad-Toh,Sante Gnerre,Michele Clamp,Jean Chang,Robert M. Stephens,Beena Neelam,Natalia Volfovsky,Alejandro A. Schäffer,Richa Agarwala,Kristina Narfström,William J. Murphy,Urs Giger,Alfred L. Roca,Agostinho Antunes,Agostinho Antunes,Marilyn Menotti-Raymond,Naoya Yuhki,Jill Pecon-Slattery,Warren E. Johnson,Guillaume Bourque,Glenn Tesler,Nisc Comparative Sequencing Program,Stephen J. O'Brien +26 more
TL;DR: The genome sequence of an inbred Abyssinian domestic cat was assembled, mapped, and annotated with a comparative approach that involved cross-reference to annotated genome assemblies of six mammals, shedding new light on the tempo and mode of gene/genome evolution in mammals and promising several research applications for the cat.
Journal ArticleDOI
Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell.
TL;DR: It is shown that virus inactivation is dependent both on the virus and on the cell line used to produce the virus, and can be correlated with the ability of the producer cells to be lysed by human serum.
References
More filters
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Mathematical model for studying genetic variation in terms of restriction endonucleases
Masatoshi Nei,Wen-Hsiung Li +1 more
TL;DR: A mathematical model for the evolutionary change of restriction sites in mitochondrial DNA is developed and a measure called "nucleotide diversity" is proposed to express the degree of polymorphism in a population at the nucleotide level.
Journal ArticleDOI
Selective extraction of polyoma DNA from infected mouse cell cultures.
Book
Walker's mammals of the world
Ronald M. Nowak,Ernest P. Walker +1 more
TL;DR: The sixth edition of Ernest P. Walker's Mammals of the World represents more than half a century of scholarship-and remains true to Walker's vision, smoothly combining thorough scholarship with a popular, readable style to preserve and enhance what the Washington Post called "a landmark of zoological literature."