Monitoring dynamic collagen reorganization during skin stretching with fast polarization-resolved second harmonic generation imaging
Guillaume Ducourthial,Jean-Sébastien Affagard,Margaux Schmeltz,Xavier Solinas,Maeva Lopez-poncelas,Christelle Bonod-Bidaud,Ruth Rubio-Amador,Florence Ruggiero,Jean-Marc Allain,Jean-Marc Allain,Emmanuel Beaurepaire,Marie-Claire Schanne-Klein +11 more
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TLDR
A fast polarization‐resolved second harmonic generation microscope is implemented to map collagen orientation during mechanical assays and successfully highlights the collagen dynamic alignment along the traction direction in ex vivo murine skin dermis.Abstract:
The mechanical properties of biological tissues are strongly correlated to the specific distribution of their collagen fibers. Monitoring the dynamic reorganization of the collagen network during mechanical stretching is however a technical challenge because it requires mapping orientation of collagen fibers in a thick and deforming sample. In this work, a fast polarization-resolved SHG microscope is implemented to map collagen orientation during mechanical assays. This system is based on line-to-line switching of polarization using an electro-optical modulator and works in epidetection geometry. After proper calibration, it successfully highlights the collagen dynamic alignment along the traction direction in ex vivo murine skin dermis. This microstructure reorganization is quantified by the entropy of the collagen orientation distribution as a function of the stretch ratio. It exhibits a linear behavior, whose slope is measured with a good accuracy. This approach can be generalized to probe a variety of dynamic processes in thick tissues.read more
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Harmonic optical tomography of nonlinear structures
Chenfei Hu,Jeffrey J. Field,Varun A. Kelkar,Benny Chiang,Keith A. Wernsing,Kimani C. Toussaint,Randy A. Bartels,Gabriel Popescu +7 more
TL;DR: This work presents harmonic optical tomography (HOT) as a novel modality for imaging microscopic, nonlinear and inhomogeneous objects and shows that this approach applies to any coherent nonlinear process.
Journal ArticleDOI
Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy.
Danielle Tokarz,Richard Cisek,Ariana Joseph,Ahmad Golaraei,Ahmad Golaraei,Kamdin Mirsanaye,Serguei Krouglov,Sylvia L. Asa,Brian C. Wilson,Brian C. Wilson,Virginijus Barzda +10 more
TL;DR: Changes in the collagen ultrastructure in pancreatic cancer were investigated by a polarization-sensitive SHG microscopy technique, and the degree of linear polarization (DOLP) of the SHG signal, which indicates the relative linear depolarization of the signal, was determined.
Journal ArticleDOI
Polarization-dependent second-harmonic generation for collagen-based differentiation of breast cancer samples.
Vassilis Tsafas,Evangelia Gavgiotaki,Maria Tzardi,Effrosyni Tsafa,Costas Fotakis,Irene Athanassakis,George Filippidis +6 more
TL;DR: The analysis presented herein suggests that PSHG imaging could provide a quantitative evaluation of the tumor state and the distinction of malignant from benign breast tissues, while potentially improving cancer detection rates.
References
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Image Analysis and Mathematical Morphology
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Journal ArticleDOI
Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure
Xiyi Chen,Oleg Nadiarynkh,Oleg Nadiarynkh,Sergey V. Plotnikov,Sergey V. Plotnikov,Paul J. Campagnola +5 more
TL;DR: This work discusses how second-harmonic generation microscopy can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other microscopy techniques.
Journal ArticleDOI
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Journal ArticleDOI
Mechanical characterization of skin—Finite deformations
D.R. Veronda,R.A. Westmann +1 more
TL;DR: Results from unaxial tests on cat skin are presented and comparing the experimentally determined force-extension curves with the analytical stress-strain relations permits the determination of suitable strain energy functions.