Nanoparticle-detained toxins for safe and effective vaccination
TLDR
It is found that the non-disruptive detoxification approach benefited the immunogenicity and efficacy of toxoid vaccines, and mice vaccinated with the nanoparticle-detained toxin showed superior protective immunity against toxin adverse effects.Abstract:
Toxoid vaccines--vaccines based on inactivated bacterial toxins--are routinely used to promote antitoxin immunity for the treatment and prevention of bacterial infections. Following chemical or heat denaturation, inactivated toxins can be administered to mount toxin-specific immune responses. However, retaining faithful antigenic presentation while removing toxin virulence remains a major challenge and presents a trade-off between efficacy and safety in toxoid development. Here, we show a nanoparticle-based toxin-detainment strategy that safely delivers non-disrupted pore-forming toxins for immune processing. Using erythrocyte membrane-coated nanoparticles and staphylococcal α-haemolysin, we demonstrate effective virulence neutralization via spontaneous particle entrapment. Compared with vaccination with heat-denatured toxin, mice vaccinated with the nanoparticle-detained toxin showed superior protective immunity against toxin-mediated adverse effects. We find that the non-disruptive detoxification approach benefited the immunogenicity and efficacy of toxoid vaccines. We anticipate that this study will open new possibilities in the preparation of antitoxin vaccines against the many virulence factors that threaten public health.read more
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References
More filters
Journal ArticleDOI
Erythrocyte membrane-camouflaged polymeric nanoparticles as a biomimetic delivery platform
TL;DR: A top-down biomimetic approach in particle functionalization is reported by coating biodegradable polymeric nanoparticles with natural erythrocyte membranes, including both membrane lipids and associated membrane proteins for long-circulating cargo delivery.
Journal ArticleDOI
Immunological properties of engineered nanomaterials.
TL;DR: Research shows that nanoparticles can stimulate and/or suppress the immune responses, and that their compatibility with the immune system is largely determined by their surface chemistry, and modifying these factors can significantly reduce the immunotoxicity of nanoparticles and make them useful platforms for drug delivery.
Journal ArticleDOI
Exploiting lymphatic transport and complement activation in nanoparticle vaccines.
Sai T. Reddy,André J. van der Vlies,Eleonora Simeoni,Veronique Angeli,Veronique Angeli,Gwendalyn J. Randolph,Conlin P. O'Neil,Leslie K. Lee,Melody A. Swartz,Jeffrey A. Hubbell +9 more
TL;DR: In this article, the authors investigate whether nanoparticles can be used as a vaccine platform by targeting lymph node-residing dendritic cells via interstitial flow and activating these cells by in situ complement activation.
Journal Article
Exploiting lymphatic transport and complement activation in nanoparticle vaccines
Sai T. Reddy,A. J. van der Vlies,Eleonora Simeoni,C. P. O"neil,Melody A. Swartz,Jeffrey A. Hubbell +5 more
TL;DR: This work investigates whether nanoparticles can be used as a vaccine platform by targeting lymph node–residing dendritic cells via interstitial flow and activating these cells by in situ complement activation, and demonstrates generation of humoral and cellular immunity in mice in a size- and complement-dependent manner.
Journal ArticleDOI
Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions
Alessandro Parodi,Nicoletta Quattrocchi,Nicoletta Quattrocchi,Anne L. van de Ven,Ciro Chiappini,Michael Evangelopoulos,Jonathan O. Martinez,Jonathan O. Martinez,Brandon S. Brown,Brandon S. Brown,Sm Z. Khaled,Iman K. Yazdi,Iman K. Yazdi,Maria Vittoria Enzo,Maria Vittoria Enzo,Lucas Isenhart,Mauro Ferrari,Ennio Tasciotti +17 more
TL;DR: It is shown that nanoporous silicon particles can successfully perform all actions when they are coated with cellular membranes purified from leukocytes, and leukolike vectors retained their functions when injected in vivo, showing enhanced circulation time and improved accumulation in a tumour.
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