Journal ArticleDOI
New opportunities in micro- and macro-attenuated total reflection infrared spectroscopic imaging: Spatial resolution and sampling versatility
K. L. A. Chan,Sergei G. Kazarian +1 more
TLDR
The results showed that a spatial resolution up to 13 μm can be achieved without the use of infrared microscope objectives, which is the first time that FT-IR imaging is reported using such a versatile accessory based on a diamond ATR crystal.Abstract:
New opportunities exist to obtain chemical images using attenuated total reflection infrared (ATR-IR) spectroscopy. This paper shows the feasibility of obtaining FT-IR images with a spatial resolution of at least 3-4 μm using a Ge ATR objective coupled with an infrared microscope. The improved spatial resolution compared to FT-IR images obtained by the transmission method is due to the high refractive index of the ATR crystal, which gives a high numerical aperture and hence, a higher spatial resolution. FT-IR imaging with a conventional diamond ATR accessory has been investigated. This is the first time that FT-IR imaging is reported using such a versatile accessory based on a diamond ATR crystal. These results showed that a spatial resolution up to 13 μm can be achieved without the use of infrared microscope objectives. One advantage of the diamond element is that it allows pressure to be applied and hence, good contact to be obtained over the whole field of view.read more
Citations
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Journal ArticleDOI
Using Fourier transform IR spectroscopy to analyze biological materials
Matthew J. Baker,Júlio Trevisan,Paul Bassan,Rohit Bhargava,Holly J. Butler,Konrad Matthew Dorling,Peter R. Fielden,Simon W. Fogarty,Nigel J. Fullwood,Kelly A. Heys,Caryn Hughes,Peter Lasch,Pierre L. Martin-Hirsch,Blessing Obinaju,Ganesh D. Sockalingum,Josep Sulé-Suso,Rebecca J. Strong,Michael J. Walsh,Bayden R. Wood,Peter Gardner,Francis Martin +20 more
TL;DR: This manuscript brings together some of the leaders in this field to allow the standardization of methods and procedures for adapting a multistage approach to a methodology that can be applied to a variety of cell biological questions or used within a clinical setting for disease screening or diagnosis.
Journal ArticleDOI
Pharmaceutical applications of vibrational chemical imaging and chemometrics: a review.
TL;DR: An overview of infrared, near-infrared and Raman imaging in pharmaceutics is given, notably as a tool for enhancing drug quality and understanding process.
Journal ArticleDOI
ATR-FTIR spectroscopic imaging: recent advances and applications to biological systems
TL;DR: This review summarises some of the recent advances and applications of ATR-FTIR spectroscopic imaging in the area of biomedical studies, including examples of section of aorta, skin tissue and live cells.
Journal ArticleDOI
Applications of ATR-FTIR spectroscopic imaging to biomedical samples
Sergei G. Kazarian,K.L.A. Chan +1 more
TL;DR: Recent advances in the applications of ATR-FTIR imaging to dissolution of pharmaceutical formulations and drug release are summarized and an opportunity to image arterial samples in contact with solutions containing drug molecules is opened.
Journal ArticleDOI
FOURIER TRANSFORM INFRARED VIBRATIONAL SPECTROSCOPIC IMAGING: Integrating Microscopy and Molecular Recognition*
Ira W. Levin,Rohit Bhargava +1 more
TL;DR: The impact and promise of this spectroscopic imaging methodology are illustrated and fundamental principles of the technique are presented in the context of FTIR spectroscopy and new applications are reviewed ranging from the physical chemistry of macromolecular systems to the detection of human disease.
References
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Micro-thermal analysis: techniques and applications
TL;DR: Micro-thermal analysis as mentioned in this paper is a technique that uses a near-field thermal probe to exploit the benefits of using thermal excitation to select regions of a solid sample by means of surface or sub-surface imaging (atomic force microscopy or scanning thermal microscopy).
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FT-IR spectroscopic investigations of single cells on the subcellular level
TL;DR: In this article, the authors applied spatially resolved Fourier transform infrared (FT-IR) spectroscopy of single oral mucosa cells to acquire structural information of cell organelles.
High spatial resolution subsurface microscopy
TL;DR: In this paper, a high-spatial-resolution subsurface microscopy technique that significantly increases the numerical aperture of a microscope without introducing an additional spherical aberration is presented. But this technique is not suitable for high-resolution image inspection of Si integrated circuits.
Journal ArticleDOI
High spatial resolution subsurface microscopy
TL;DR: In this paper, a high-spatial-resolution subsurface microscopy technique that significantly increases the numerical aperture of a microscope without introducing an additional spherical aberration is presented. But this technique is not suitable for high-resolution image inspection of Si integrated circuits.
Journal ArticleDOI
Realization of numerical aperture 2.0 using a gallium phosphide solid immersion lens
TL;DR: In this paper, a gallium phosphide, hemispherical, solid immersion lens was used to image 40-nm-diam fluorescent dye balls at a wavelength of 560 nm, which is equivalent to a diffraction-limited system of numerical aperture 2.0.