Showing papers in "Journal of Molecular Biology in 1983"

TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.

TL;DR: A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.

TL;DR: The complete nucleotide sequence of bacteriophage T7 DNA, 39,936 base-pairs, has been determined and the amino acid sequences and compositions predicted for all of the T7 proteins (except the proteins produced by frameshifting) are given.

TL;DR: The proposed existence of a signal peptidase recognition sequence A-X-B with the preferred cleavage site located after the sixth amino acid following the core sequence is proposed.

TL;DR: "Pseudo-structures" of the 20 common amino acid residues are introduced for use in protein spatial structure determinations, which rely on the use of intramolecular proton-proton distance constraints determined by nuclear Overhauser effects as input for distance geometry calculations.

TL;DR: The thickness of the lipid bilayer in vesicles made of pure phosphatidylcholines, with acyl chain lengths ranging from 10 to 24 carbons, has been determined by analysis of continuous X-ray scattering data from vesicle pellets at temperatures above the lipid phase transition temperature.

TL;DR: The results obtained indicate that on average one-third of the charged residues in a protein are involved in ion-pairs and 76% of these are concerned with stabilizing the tertiary (rather than the secondary) structure.

TL;DR: A chromosomal walk is described that covers 315 X 10(3) base-pairs of DNA from the 87DE region of the third chromosome of Drosophila melanogaster, which includes the DNA for the rosy and Ace loci, which code for xanthine dehydrogenase and acetylcholinesterase.

TL;DR: The overall quaternary structure of oxyhaemoglobin is identical, within experimental error, to that of carbon monoxide haemoglobin, and thus confirms the applicability of the allosteric mechanisms proposed by Perutz and Baldwin & Chothia to the process of oxygen binding.

TL;DR: A general method of assigning the non-exchangeable protons in the nuclear magnetic resonance spectra of small DNA molecules has been developed based upon two-dimensional autocorrelated (COSY) and nuclear Overhauser (NOESY) spectra in 2H2O solutions.

TL;DR: A number of features of the molecular structure appear to be directly related to the function of plastocyanin as an electron carrier in photosynthesis, and are unlikely to be connected with structural changes in the oxidized form of the protein.

TL;DR: The specificity subunits, though components of a family of closely related enzymes with very similar functions, have remarkably dissimilar primary structure.

TL;DR: Within these limits, the sequence-derived sum functions match the observed helix parameter variation quite closely, with correlation coefficients greater than 0.900 in nearly all cases.

TL;DR: The crystal structure of the double-helical B-DNA dodecamer has been solved and refined independently in three forms and the zig-zag spine of hydration that the authors believe to be principally responsible for stabilizing the B form of DNA is found in all three structures.

TL;DR: Penicillopepsin has had its molecular structure refined by a restrained-parameter least-squares procedure at 1.8 A resolution to a conventional R -factor of 0.136, confirming the proposal that conformational mobility of the active site residues has no role in the enzymatic mechanism.

TL;DR: By considering the observed frequency of amino acid replacements among related structures, a set of optimal matching hydrophobicities (OMHs) was derived and significant correlation coefficients are calculated for similar three-dimensional structures, even though the two sequences contain few identical residues.

TL;DR: In this paper, a series of 12 DNA molecules has been studied, which range in length from 237 to 254 bp with a period of about 10 bp, and it was shown that the DNA twisting potential is symmetric.

TL;DR: In this paper, the motion of the atoms in the small protein bovine pancreatic trypsin inhibitor has been simulated for about 60 picoseconds using two different potential energy functions.

TL;DR: The crystal structure of bovine carboxypeptidase A (Cox) has been refined at 1.54 A resolution using the restrained least-squares algorithm of Hendrickson & Konnert (1981) and four examples of a reverse turn like structure requiring an aspartic acid or asparagine residue are observed in this structure.

TL;DR: Native-like folded conformations of bovine pancreatic trypsin inhibitor protein are calculated by searching for conformations with the lowest possible potential energy, and there is a relationship between the root-mean-square deviation and the energy, in that the lowest energy conformations are also closest to the X-ray structure.

TL;DR: The absence of long range nuclear Overhauser effects between the peptide segments 5-9 and 11-29, and between 5-16 and 19-29 shows that the polypeptide chain does not fold back on itself and hence that micelle-bound glucagon does not adopt a globular tertiary structure.

TL;DR: Molecular models suggest that the coiled coil is continuous throughout the rod, with an approximately uniform left-handed twist, except for a few turns of helix near each skip region, where the twist flattens out to accommodate the extra residue.

TL;DR: In this paper, the X-ray structure analysis and refinement at 1·9 A resolution of calf γ-II crystallin, a lens-specific protein, has been reported, which has a symmetrical, hierarchical structure of two globular domains each comprising two similar “Greek key” motifs, consecutive along the polypeptide chain, and related by a pseudo 2-fold axis.

TL;DR: Through a combination of subcloning and Tn1000 mutagenesis, a region of 2.2 X 10(3) bases which contains the information necessary to complement umuC mutations is identified which is essential for "error-prone repair" in E. coli.

TL;DR: Using a lacI-Z fusion system, the efficiency of suppression of nonsense codons in the I gene of Escherichia coli is determined by assaying beta-galactosidase activity and models explaining the effect of the surrounding sequence on suppression efficiency are considered.

TL;DR: Comparison of the two results using the simplest kinetic model suggests that the 30-fold more rapid overall association rate for the reaction of oxygen with myoglobin compared to carbon monoxide results mainly from faster binding at the heme, with a small contribution from more rapid entry of oxygen into the protein from the solvent.

TL;DR: Using desmoplakin antibodies, Desmoplakins are identified as the major constituents of the desmosomal plaques present in epithelial and myocardiac cells of diverse species.

TL;DR: The value of 2·5 myosins per 14·3 nm repeat as calculated from these results suggests that thick filament models with mixtures of two and three crossbridges per repeat should be considered.

TL;DR: It is discussed the possibility that a loss of keratin binding ability, resulting in a loosening of the keratin fibre/filaggrin matrix is necessary before the normal complete proteolysis of the filaggrins can occur.

TL;DR: The investigation shows that, in contrast to the previous report, the three-dimensional structure of porcine phospholipase A2 is very similar to that of bovine phosphate A2, including the active site, and an appreciable conformational difference is in the loop 59 to 70.