Journal ArticleDOI
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
F W Studier,B A Moffatt +1 more
TLDR
A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.About:
This article is published in Journal of Molecular Biology.The article was published on 1986-05-05. It has received 6415 citations till now. The article focuses on the topics: T7 RNA polymerase & RNA-dependent RNA polymerase.read more
Citations
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Book ChapterDOI
Use of T7 RNA polymerase to direct expression of cloned genes.
Journal ArticleDOI
Green fluorescent protein as a marker for gene expression
TL;DR: A complementary DNA for the Aequorea victoria green fluorescent protein produces a fluorescent product when expressed in prokaryotic or eukaryotic cells, which can be used to monitor gene expression and protein localization in living organisms.
Journal ArticleDOI
Protein production by auto-induction in high-density shaking cultures
TL;DR: Investigation of factors that affect stability, growth, and induction of T7 expression strains in shaking vessels led to the recognition that sporadic, unintended induction of expression in complex media, previously reported by others, is almost certainly caused by small amounts of lactose.
Journal ArticleDOI
Cellular uptake of the tat protein from human immunodeficiency virus
Alan D. Frankel,Carl O. Pabo +1 more
TL;DR: Experiments using radioactive protein show that tat becomes localized to the nucleus after uptake and suggest that chloroquine protects tat from proteolytic degradation, raising the possibility that, under some conditions, tat might act as a viral growth factor to stimulate viral replication in latently infected cells or alter expression of cellular genes.
Journal ArticleDOI
Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase
TL;DR: The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Analysis of bacteriophage T7 early RNAs and proteins on slab gels
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.
Journal ArticleDOI
Compilation and analysis of Escherichia coli promoter DNA sequences.
TL;DR: A consensus promoter sequence based on homologies among 112 well-defined promoters was determined that was in substantial agreement with previous compilations.
Journal ArticleDOI
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
TL;DR: Comparison of the sequence of λrifd18 with sequences from other isolates of the rrB operon provides direct evidence for structural rearrangements within rRNA operons.
Journal ArticleDOI
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
TL;DR: The complete nucleotide sequence of bacteriophage T7 DNA, 39,936 base-pairs, has been determined and the amino acid sequences and compositions predicted for all of the T7 proteins (except the proteins produced by frameshifting) are given.