Journal ArticleDOI
New substrates for the fluorometric determination of oxidative enzymes.
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This article is published in Analytical Chemistry.The article was published on 1967-02-01. It has received 393 citations till now. The article focuses on the topics: Oxidative enzyme.read more
Citations
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Journal ArticleDOI
Generation of reactive oxygen species by the mitochondrial electron transport chain
TL;DR: In this paper, it was shown that the major ROS-generating site in mitochondria is limited to the flavin mononucleotide group (FMN) of complex I through reversed electron transfer, not at the ubiquinone of complex III.
Journal ArticleDOI
Photometric method for the determination of low concentrations of hydrogen peroxide by the peroxidase catalyzed oxidation of N,N-diethyl-p-phenylenediamine (DPD)
TL;DR: In this paper, the concentration of hydrogen peroxide (H2O2) in distilled water, drinking water and in different types of surface and rain waters can be easily determined by a photometric method in which N,N-diethyl-p-phenylenediamine (DPD) is oxidized by a peroxidase catalyzed reaction.
Journal ArticleDOI
The Regulation of Reactive Oxygen Species Production during Programmed Cell Death
TL;DR: It is found that GSH depletion is not sufficient to cause the maximal mitochondrial ROS production, and that there is an early requirement for protease activation, changes in gene expression, and a late requirement for Ca2+ mobilization.
Journal ArticleDOI
Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.
TL;DR: Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described, permitting one person to carry out analyses of 30 or more subcultures in one day.
Journal ArticleDOI
The picomole determination of free and total cholesterol in cells in culture.
J G Heider,R L Boyett +1 more
TL;DR: It is rapid, in that free cholesterol can be read in 5 minutes and total cholesterol after 20 minutes, and the precision of the method is greater than that obtained from gas-liquid chromatography.
References
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Book ChapterDOI
The assay of catalases and peroxidases.
Britton Chance,A.C. Maehly +1 more
TL;DR: Two methods are described for the catalase assay by disappearance of peroxide are: ultraviolet spectrophotometry and permanganate titration and indirect measurements of the decrease of light absorption caused by the decomposition of hydrogen peroxide byCatalase.
Journal ArticleDOI
The fluorometric analysis of ultramicro quantities of hydrogen peroxide.
TL;DR: A fluorometric method is described for the analysis of hydrogen peroxide in the range of 10 −11 mole/ml based on the oxidation of nonfluorescent diacetyldichlorofluorescin to a fluorescent compound by hydrogenperoxide and peroxidase.
Journal ArticleDOI
The D-galactose oxidase of Polyporus circinatus.
TL;DR: The mold Polyporus circinatus produces an extracellular oxidase that catalyzes the oxidation of n-galactose, but the enzyme does not possess a flavin prosthetic group, and preliminary evidence suggests that it may be a metalloprotein.
Journal ArticleDOI
A Sensitive Method for the Estimation of Hydrogen Peroxide in Biological Materials
TL;DR: The method of detecting hydrogen peroxide to be described is based on the same principle but has the advantage of greater sensitivity and the intensity of fluorescence is proportional to the concentration of scopoletin.