Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.
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TLDR
Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described, permitting one person to carry out analyses of 30 or more subcultures in one day.About:
This article is published in Journal of Lipid Research.The article was published on 1978-11-01 and is currently open access. It has received 555 citations till now. The article focuses on the topics: Cholesterol oxidase.read more
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The Surface of Lipid Droplets Is a Phospholipid Monolayer with a Unique Fatty Acid Composition
TL;DR: The result indicates that the lipid droplet surface is a hemi-membrane or a phospholipid monolayer containing cholesterol but is compositionally different from the endoplasmic reticulum membrane or the sphingolipids/cholesterol-rich microdomain.
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Cholesterol is required for surface transport of influenza virus hemagglutinin
Patrick Keller,Kai Simons +1 more
TL;DR: These data provide for the first time direct evidence for the functional significance of cholesterol in the sorting of apical membrane proteins as well as of apically secreted glycoproteins.
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Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-methyltetrahydrofolate.
TL;DR: It is found that cholesterol plays a critical role in maintaining the caveola membrane domain and modulates the interaction of GPI-anchored membrane proteins via their phospholipid anchors.
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Macrophage oxidation of low density lipoprotein generates a modified form recognized by the scavenger receptor.
TL;DR: The degradation of 125l-labeled macrophage-modified LDL by macrophages was competitively inhibited by unlabeled acetyl LDL or unlabeling endothelial cell-modified HDL but not by native LDL, indicating that the degradation was mediated by the acetyl HDL receptor.
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Enhanced macrophage degradation of biologically modified low density lipoprotein.
TL;DR: A biologically generated modification of LDL is described that markedly alters cholesterol metabolism of macrophages and, consequently, may play a role in foam cell formation during atherogenesis.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Enzymatic determination of total serum cholesterol.
TL;DR: An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent and has excellent precision.
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Amino Acid Metabolism in Mammalian Cell Cultures
TL;DR: The present article "is a progress report rather than a review and in large part summarizes studies from a single laboratory" on the minimal essential medium for cultivation of mammalian cells in either monolayer or suspension.
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The picomole determination of free and total cholesterol in cells in culture.
J G Heider,R L Boyett +1 more
TL;DR: It is rapid, in that free cholesterol can be read in 5 minutes and total cholesterol after 20 minutes, and the precision of the method is greater than that obtained from gas-liquid chromatography.