Journal ArticleDOI
NMR analysis of mono- and difructosyllactosucrose synthesized by 1F-fructosyltransferase purified from roots of asparagus (Asparagus officinalis L.)
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TLDR
In this paper, two novel oligosaccharides, mono-and difructosyllactosucrose, were synthesized using 1F-fructosyltransferase purified form roots of asparagus (Asparagus officinalis L.).Abstract:
Two novel oligosaccharides, mono- and difructosyllactosucrose {[O-β-D-fructofuranosyl-(2 1)]n-β-D-fructofuranosyl-O-[β-D-galactopyranosyl-(1 4)]-α-D-glucopyranoside, n = 1 and 2} were synthesized using 1F-fructosyltransferase purified form roots of asparagus (Asparagus officinalis L.). Their 1H and 13C NMR spectra were assigned using several NMR techniques. The spectral analysis was started from two anomeric methines of aldose units, galactose and glucose, since they showed separate characteristic signals in their 1H and 13C NMR spectra. After assignments of all the 1H and 13C signals of two units of aldose, they were discriminated as galactose and glucose using proton–proton coupling constants. The HMBC spectrum revealed the galactose residue attached to C-4 of glucose, fructose residue attached to the C-1 of glucose, and further fructosyl fructose linkage extended from the glucosyl fructose residues. Copyright © 2002 John Wiley & Sons, Ltd.read more
Citations
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Journal ArticleDOI
Fructooligosaccharides and fructans analysis in plants and food crops
TL;DR: This paper aims to review and describe the different chromatographic techniques and other methods that are used to separate, analyze and quantify FOS and fructans.
Journal ArticleDOI
Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis.
Woo-Cheul Han,Sun-Ho Byun,Mi-Hyun Kim,Eun Hwa Sohn,Jung Dae Lim,Byung-Hun Um,Chul Ho Kim,Soon Ah Kang,Ki-Hyo Jang +8 more
TL;DR: A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as result.
Journal ArticleDOI
Novel fructopyranose oligosaccharides isolated from fermented beverage of plant extract
Hideki Okada,Eri Fukushi,Akira Yamamori,Naoki Kawazoe,Shuichi Onodera,Jun Kawabata,Norio Shiomi +6 more
TL;DR: Four oligosaccharides containing a fructopyranosyl residue have been found from fermented beverage of plant extract and isolated from the beverage using carbon-Celite column chromatography and preparative high performance liquid chromatography.
Journal ArticleDOI
Advanced NMR Approaches for a Detailed Structure Analysis of Natural Products
TL;DR: Selective detection of methine, methylene, or methylene signals in each subspectrum by editing NMR methods was utilized to reduce the complexity in crowded spectra and enhance the sensitivity by limiting the measuring area of the 2D spectra.
Journal ArticleDOI
Efficient Biosynthesis of Lactosucrose from Sucrose and Lactose by the Purified Recombinant Levansucrase from Leuconostoc mesenteroides B-512 FMC
TL;DR: Under the optimized reaction conditions, the maximal lactosucrose yield reached 224 g L(-1) after reaction for 1 h, and L. mesenteroides levansucrase could be considered a potential candidate for future industrial production of lactosUCrose.
References
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TL;DR: New NMR pulse sequences CT-HMBC-1 and CT-HBC-2 which incorporate constant time evolution period are more useful than the conventional HMBC technique for improving separation of cross peaks.
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Properties of Fructosyltransferases Involved in the Synthesis of Fructan in Liliaceous Plants
TL;DR: From the properties and substrate specificities of SST, 6 G -FT and 1 F -FT, a scheme for the biosyntheticpathways of fructans in asparagus and onion plants was proposed.