Journal ArticleDOI
Nonlinear scanning laser microscopy by third harmonic generation
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In this article, the authors used the third harmonic generation near the focal point of a tightly focused beam to probe microscopical structures of transparent samples, which can resolve interfaces and inhomogeneities with axial resolution comparable to the confocal length of the beam.Abstract:
Third harmonic generation near the focal point of a tightly focused beam is used to probe microscopical structures of transparent samples. It is shown that this method can resolve interfaces and inhomogeneities with axial resolution comparable to the confocal length of the beam. Using 120 fs pulses at 1.5 μm, we were able to resolve interfaces with a resolution of 1.2 μm. Two-dimensional cross-sectional images have also been produced.read more
Citations
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Nonlinear magic: multiphoton microscopy in the biosciences
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Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
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In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Nicholas G. Horton,Ke Wang,Demirhan Kobat,Catharine G. Clark,Frank W. Wise,Chris B. Schaffer,Chris Xu +6 more
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Two-Photon Excitation Fluorescence Microscopy
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References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Book
The Principles of Nonlinear Optics
TL;DR: In this article, the authors present a general description of wave propagation in nonlinear media, including high-resolution nonlinear optical spectroscopy, and four-wave mixing and mixing.
Journal ArticleDOI
Optical third-harmonic generation at interfaces
TL;DR: It is reported that when using focused high-intensity ultrashort laser pulses, this normally weak THG process becomes highly operative at a simple air-dielectric interface and is much stronger than the bulk of most dielectric materials.
Journal ArticleDOI
Second-harmonic imaging in the scanning optical microscope
TL;DR: In this paper, a scanning optical microscope in which an image is produced from the generation of optical second harmonics within the specimen has been constructed, and pictures have been obtained from various crystals which show high contrast levels and detail not visible with the conventional microscope.
Journal ArticleDOI
Nonlinear optical microscopic examination of structure in polycrystalline ZnSe
TL;DR: In this paper, the spatial variations in the second-order nonlinear susceptibility of a ZnSe polycrystal were used to generate images of its physical structure at the second harmonic frequency (532 nm) of an incident 1.06 μm laser beam.