Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
Reads0
Chats0
TLDR
Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.Abstract:Â
Contrary to the well known diffraction limit, the fluorescence microscope is in principle capable of unlimited resolution. The necessary elements are spatially structured illumination light and a nonlinear dependence of the fluorescence emission rate on the illumination intensity. As an example of this concept, this article experimentally demonstrates saturated structured-illumination microscopy, a recently proposed method in which the nonlinearity arisesfromsaturationoftheexcitedstate.Thismethodcanbeused in a simple, wide-field (nonscanning) microscope, uses only a single, inexpensive laser, and requires no unusual photophysical properties of the fluorophore. The practical resolving power is determined by the signal-to-noise ratio, which in turn is limited by photobleaching. Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples.read more
Citations
More filters
Book ChapterDOI
Single molecule tracking for studying nucleocytoplasmic transport and intranuclear dynamics.
TL;DR: Single-molecule fluorescence microscopy provides a molecular, real-time view of physiological processes in vivo and is an ideal tool to study intranuclear protein or ribonucleoprotein particle mobility.
Patent
Systems, methods, apparatus and computer-accessible-medium for providing polarization-mode dispersion compensation in optical coherence tomography
Benjamin J. Vakoc,Ziyi Zhang +1 more
TL;DR: In this article, it is shown that it is possible to generate second data by reducing the influence of first optical effects induced on the first radiation prior to reaching the sample(s) and second optical effects inducing on the second radiation after leaving the sample (s).
Journal ArticleDOI
Exceeding the limits of 3D fluorescence microscopy using a dual-stage-processing network
TL;DR: A dual-stage mutual-feedback deep-learning approach that allows gradual reversion of microscopy degradation from high-resolution targets to low-resolution images, using a single blurred-and-pixelated 3D image as input and infers a 3D output with notably higher resolution and improved contrast is reported.
Book ChapterDOI
Structural Plasticity in Dendrites: Developmental Neurogenetics, Morphological Reconstructions, and Computational Modeling
TL;DR: Time-lapse confocal images of the fluorescently labeled cytoskeletal components are digitally reconstructed into a novel file structure, enabling comprehensive statistical analysis and data-driven computational simulations of dendritic growth.
Journal ArticleDOI
Deep learning autofluorescence-harmonic microscopy
TL;DR: Li et al. as mentioned in this paper proposed a self-alignment attention-guided residual-in-residual dense generative adversarial networks (DLAM) to close the gap between imaging speed, field-of-view (FOV), and spatial resolution.
References
More filters
Journal ArticleDOI
Introduction to Fourier Optics
Joseph W. Goodman,Mary E. Cox +1 more
TL;DR: The second edition of this respected text considerably expands the original and reflects the tremendous advances made in the discipline since 1968 as discussed by the authors, with a special emphasis on applications to diffraction, imaging, optical data processing, and holography.
Book
Introduction to Fourier optics
TL;DR: The second edition of this respected text considerably expands the original and reflects the tremendous advances made in the discipline since 1968 as discussed by the authors, with a special emphasis on applications to diffraction, imaging, optical data processing, and holography.
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Semiconductor Nanocrystals as Fluorescent Biological Labels
TL;DR: Semiconductor nanocrystals prepared for use as fluorescent probes in biological staining and diagnostics have a narrow, tunable, symmetric emission spectrum and are photochemically stable.
Book
The Principles of Nonlinear Optics
TL;DR: In this article, the authors present a general description of wave propagation in nonlinear media, including high-resolution nonlinear optical spectroscopy, and four-wave mixing and mixing.
Related Papers (5)
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more