Journal ArticleDOI
Optical Anisotropy of Supported Lipid Structures Probed by Waveguide Spectroscopy and Its Application to Study of Supported Lipid Bilayer Formation Kinetics
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TLDR
Understanding of mechanistic details for an adsorption process for which conformational changes and ordering occur can be elucidated using DPI and greatly enhanced by modeling of optical birefringence.Abstract:
Supramolecular conformation and molecular orientation was monitored during supported lipid bilayer (SLB) formation using dual polarization interferometry (DPI). DPI was shown to enable real time sensitive determination of birefringence of the lipid bilayer together with thickness or refractive index (with the other a fixed value). This approach removes differences in mass loading due to anisotropy, so the mass becomes solely a function of the lipid dn/dc value. DPI measurements show highly reproducible qualitative and quantitative results for adsorption of liposomes of different lipid compositions and in buffers with or without CaCl2. The packing of solvent-free self-assembled SLBs is shown to differ from other preparation methods. Birefringence analysis accompanied by mass and thickness measurements shows characteristic features of vesicle adsorption and SLB formation kinetics previously not demonstrated by evanescent optical techniques, including indications of percolation-type rupture of clusters of li...read more
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Dissertation
Développement de chimie de surface pour la réduction de l’adsorption non-spécifique de lysat cellulaire et application clinique de biocapteurs SPR
TL;DR: Peptide and ionic liquid monolayers have been studied in this thesis to prevent nonspecific adsorption of undiluted cell lysate and it was proved that the ionic liquids could be used in the development of clinical biosensors.
Journal ArticleDOI
Time-Resolved Thickness and Shape-Change Quantification using a Dual-Band Nanoplasmonic Ruler with Sub-Nanometer Resolution
Ferry Anggoro Ardy Nugroho,Dominika Świtlik,Antonius Armanious,Padraic O’Reilly,Iwan Darmadi,Sara Nilsson,Vladimir P. Zhdanov,Fredrik Höök,Tomasz J. Antosiewicz,Christoph Langhammer +9 more
TL;DR: In this article, a dual-band nanoplasmonic ruler is presented for real-time simultaneous measurements of thickness and refractive index variations in uniform and heterogeneous layers with sub-nanometer resolution.
Book ChapterDOI
Preparation and Characterization of Supported Lipid Bilayers for Biomolecular Interaction Studies by Dual Polarization Interferometry
TL;DR: This multiplex analysis of the dynamic changes in lipid organization and molecular mass has provided new insight into the molecular mechanisms of membrane-active peptides and proteins especially in relation to changes in bilayer structure associated with the biomembrane binding and disruption.
Journal ArticleDOI
Thickness determination in anisotropic media with plasmon waveguide resonance imaging.
TL;DR: Good agreement between atomic force microscopy and optical measurements of thickness variation is shown, on an azobenzene modified polymer layer, and it is shown that this procedure discriminates between isotropic and anisotropic regions.
References
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Journal ArticleDOI
Structure of lipid bilayers
TL;DR: The uncertainty in structural results for lipid bilayers is being reduced and best current values are provided for bilayers of five lipids.
Journal ArticleDOI
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
TL;DR: The application of ellipsometry for the study of the adsorption behavior of proteins and synthetic macromolecules at the air-water interface has been investigated in this article.
Journal ArticleDOI
Quartz crystal microbalance setup for frequency and Q‐factor measurements in gaseous and liquid environments
TL;DR: In this paper, an experimental setup has been constructed for simultaneous measurements of the frequency, the absolute Q factor, and the amplitude of oscillation of a quartz crystal microbalance (QCM).
Journal ArticleDOI
Formation of solid-supported lipid bilayers: an integrated view.
TL;DR: The results suggest that the molecular-level interaction between lipids and the solid support needs to be considered explicitly, to understand the rupture of vesicles and the formation of SLBs as well as to predict the properties of the resulting SLB.
Journal ArticleDOI
Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance.
C.A. Keller,Bengt Herbert Kasemo +1 more
TL;DR: This work measures the kinetics of adsorption of small (12.5-nm radius) unilamellar vesicles onto SiO2, oxidized gold, and a self-assembled monolayer of methyl-terminated thiols, using a quartz crystal microbalance (QCM).
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Formation of solid-supported lipid bilayers: an integrated view.
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