Journal ArticleDOI
Optical Anisotropy of Supported Lipid Structures Probed by Waveguide Spectroscopy and Its Application to Study of Supported Lipid Bilayer Formation Kinetics
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TLDR
Understanding of mechanistic details for an adsorption process for which conformational changes and ordering occur can be elucidated using DPI and greatly enhanced by modeling of optical birefringence.Abstract:
Supramolecular conformation and molecular orientation was monitored during supported lipid bilayer (SLB) formation using dual polarization interferometry (DPI). DPI was shown to enable real time sensitive determination of birefringence of the lipid bilayer together with thickness or refractive index (with the other a fixed value). This approach removes differences in mass loading due to anisotropy, so the mass becomes solely a function of the lipid dn/dc value. DPI measurements show highly reproducible qualitative and quantitative results for adsorption of liposomes of different lipid compositions and in buffers with or without CaCl2. The packing of solvent-free self-assembled SLBs is shown to differ from other preparation methods. Birefringence analysis accompanied by mass and thickness measurements shows characteristic features of vesicle adsorption and SLB formation kinetics previously not demonstrated by evanescent optical techniques, including indications of percolation-type rupture of clusters of li...read more
Citations
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Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates
TL;DR: A protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and a recently developed strategy that uses an amphipathic, α-helical (AH) peptide to form SLBs on gold and Titanium oxide substrates are presented.
Journal ArticleDOI
Theory and applications of surface plasmon resonance, resonant mirror, resonant waveguide grating, and dual polarization interferometry biosensors.
TL;DR: This review discusses the basic theory and operational arrangements of four commercially available types of optical biosensors: surface plasmon resonance, resonant mirror, resonance waveguide grating, and dual polarization interferometry.
Journal ArticleDOI
Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rebecca L. Rich,David G. Myszka +1 more
TL;DR: This work grades every paper published in 2008 on a scale from A to F and outlines what features make a biosensor article fabulous, middling or abysmal and focuses on a few experimental, analysis and presentation mistakes that are alarmingly common.
Journal ArticleDOI
Filamentous Bacteriophage Promote Biofilm Assembly and Function
Patrick R. Secor,Johanna M. Sweere,Lia A. Michaels,Andrey V. Malkovskiy,Daniel Lazzareschi,Ethan Katznelson,Jayakumar Rajadas,Michael E. Birnbaum,Allison Arrigoni,Kathleen R. Braun,Stephen P. Evanko,David A. Stevens,Werner Kaminsky,Pradeep K. Singh,William C. Parks,Paul L. Bollyky +15 more
TL;DR: It is found that the extracellular matrix produced by Pseudomonas aeruginosa self-assembles into a liquid crystal through entropic interactions between polymers and filamentous Pf bacteriophages, which are long, negatively charged filaments, which enhance biofilm function by increasing adhesion and tolerance to desiccation and antibiotics.
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α-Synuclein Senses Lipid Packing Defects and Induces Lateral Expansion of Lipids Leading to Membrane Remodeling
Myriam Ouberai,Juan Wang,Marcus J. Swann,Céline Galvagnion,Tim Guilliams,Christopher M. Dobson,Mark E. Welland +6 more
TL;DR: The ability of α-Syn to sense lipid packing defects and to remodel membrane structure supports its proposed role in vesicle trafficking.
References
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Journal ArticleDOI
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Intact Vesicle Adsorption and Supported Biomembrane Formation from Vesicles in Solution: Influence of Surface Chemistry, Vesicle Size, Temperature, and Osmotic Pressure†
TL;DR: In this paper, the adsorption kinetics of small unilamellar egg-yolk phosphatidylcholine vesicles were investigated by the quartz crystal microbalance−dissipation (QCM−D) technique, as a function of surface chemistry (on SiO2, Si3N4, Au, TiO2 and Pt), temperature (273−303 K), vesicle size (25−200 nm), and osmotic pressure.
Journal ArticleDOI
Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study
TL;DR: Calcium was shown to enhance the tendency of SLB formation for negatively charged and zwitterionic vesicles, and the role of vesicle-support, interbilayer, and intrabilayer interactions in the formation of SLBs is discussed.
Journal ArticleDOI
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