Optical transfection system using pulse laser for massively parallel localized intracellular delivery
01 Dec 2017-pp 1-3
TL;DR: An optical setup for illuminating ns-pulse light into cells and measuring energy density to evaluate whether cell membranes can be pierced to obtain uniform cells with stable gene expression is assembled.
Abstract: Cells are the core of regenerative medicine based on iPS cells and cell therapy. These realizations are highly expected in our society. In the initial stage of cell processing, it is required to obtain uniform cells with stable gene expression. We assembled an optical setup for illuminating ns-pulse light into cells. We measured energy density to evaluate whether cell membranes can be pierced. Titanium was vapor deposited on a glass substrate. Pulse laser was irradiated on the sample to observe the change in the Ti surface.
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TL;DR: This review article will emphasize the basic concept and working mechanism associated with electroporation, single cell Electroporation and biomolecular delivery using micro/nanofluidic devices, their fabrication, working principles and cellular analysis with their advantages, limitations, potential applications and future prospects.
Abstract: © 2018 IOP Publishing Ltd. The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics and drug delivery towards personalized medicine. Many chemical and physical methods have been developed for cellular delivery, however most of these techniques are bulk approach, which are cell-specific and have low throughput delivery. On the other hand, electroporation is an efficient and fast method to deliver exogenous biomolecules such as DNA, RNA and oligonucleotides into target living cells with the advantages of easy operation, controllable electrical parameters and avoidance of toxicity. The rapid development of micro/nanofluidic technologies in the last two decades, enables us to focus an intense electric field on the targeted cell membrane to perform single cell micro-nano-electroporation with high throughput intracellular delivery, high transfection efficiency and cell viability. This review article will emphasize the basic concept and working mechanism associated with electroporation, single cell electroporation and biomolecular delivery using micro/nanoscale electroporation devices, their fabrication, working principles and cellular analysis with their advantages, limitations, potential applications and future prospects.
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