Journal ArticleDOI
Osteogenic Differentiation of Marrow Stromal Cells on Random and Aligned Electrospun Poly(l-lactide) Nanofibers
TLDR
The results indicate that BMS cells aligned in the direction of PLLA fibers to form long cell extensions, and fiber orientation affected the extent of mineralization, but it had no effect on cell proliferation or mRNA expression of osteogenic markers.Abstract:
The fibrillar structure and sub-micron diameter of electrospun nanofibers can be used to reproduce the morphology and structure of the natural extracellular matrix (ECM). The objective of this work was to investigate the effect of fiber alignment on osteogenic differentiation of bone marrow stromal (BMS) cells. Random and aligned poly(l-lactide) (PLLA) nanofibers were produced by collecting the spun fibers on a stationary plate and a rotating wheel, respectively, as the ground electrode. Morphology and alignment of the BMS cells seeded on the fibers were characterized by SEM. The effect of fiber orientation on osteogenic differentiation of BMS cells was determined by measuring alkaline phosphatase (ALPase) activity, calcium content, and mRNA expression levels of osteogenic markers. There was a strong correlation between the fiber and cell distributions for the random (p = 0.16) and aligned (p = 0.81) fibers. Percent deviation from ideal randomness (PDIR) values indicated that cells seeded on the random fibers (PDIR = 6.5%) were likely to be distributed randomly in all directions while cells seeded on the aligned fibers (PDIR = 86%) were highly likely to be aligned with the direction of fibers. BMS cell seeded on random and aligned fibers had similar cell count and ALPase activity with incubation time, but the calcium content on aligned fibers was significantly higher after 21 days compared to that of random fibers (p = 0.003). Osteopontin (OP) and osteocalcin (OC) expression levels of BMS cells on fibers increased with incubation time. However, there was no difference between the expression levels of OP and OC on aligned vs. random fibers. The results indicate that BMS cells aligned in the direction of PLLA fibers to form long cell extensions, and fiber orientation affected the extent of mineralization, but it had no effect on cell proliferation or mRNA expression of osteogenic markers.read more
Citations
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Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants.
TL;DR: The interaction of cells and tissues with artificial materials designed for applications in biotechnologies and in medicine is governed by the physical and chemical properties of the material surface, including surface roughness and surface topography.
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Gold nanoparticles promote osteogenic differentiation of mesenchymal stem cells through p38 MAPK pathway.
TL;DR: Investigation of the cellular effects of gold nanoparticles (AuNPs) on the differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms showed that AuNPs promoted the differentiate of MSCs toward osteoblast cells over adipocyte cells by inducing an enhanced osteogenic transcriptional profile and an attenuated adipogenic transcriptionAL profile.
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The guidance of stem cell differentiation by substrate alignment and mechanical stimulation.
Siddarth D. Subramony,Booth R. Dargis,Mario Castillo,Evren U. Azeloglu,Michael S. Tracey,Amanda Su,Helen H. Lu +6 more
TL;DR: Results of this study reveal that systemic and readily controllable cues are sufficient to drive MSC differentiation, without the need for additional chemical stimuli, and yield a set of fundamental design rules that can be readily applied to connective tissue regeneration strategies.
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Nanomaterial scaffolds for stem cell proliferation and differentiation in tissue engineering.
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Bioprinting of growth factors onto aligned sub-micron fibrous scaffolds for simultaneous control of cell differentiation and alignment.
Elmer D.F. Ker,Amrinder S. Nain,Lee E. Weiss,Ji Wang,Joseph Suhan,Cristina H. Amon,Phil G. Campbell +6 more
TL;DR: Functionalizing oriented sub-micron fibers with printed GFs provides instructive cues to spatially control cell fate and alignment to mimic native tissue organization and may have applications in regenerative medicine.
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