Journal ArticleDOI
Overexpression of a tea flavanone 3-hydroxylase gene confers tolerance to salt stress and Alternaria solani in transgenic tobacco.
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Overexpression of CsF3H cDNA increased the content of flavan-3-ols in tobacco and conferred tolerance to salt stress and fungus Alternaria solani infection to transgenic tobacco through improved antioxidant system and enhanced pectin methyl esterification.Abstract:
Flavan-3-ols are the major flavonoids present in tea (Camellia sinensis) leaves. These are known to have antioxidant and free radical scavenging properties in vitro. Flavanone 3-hydroxylase is considered to be an important enzyme of flavonoid pathway leading to accumulation of flavan-3-ols in tea. Expression analysis revealed the upregulation in transcript levels of C. sinensis flavanone 3-hydroxylase (CsF3H) encoding gene under salt stress. In this study, the biotechnological potential of CsF3H was evaluated by gene overexpression in tobacco (Nicotiana tabacum cv. Xanthi). Overexpression of CsF3H cDNA increased the content of flavan-3-ols in tobacco and conferred tolerance to salt stress and fungus Alternaria solani infection. Transgenic tobaccos were observed for increase in primary root length, number of lateral roots, chlorophyll content, antioxidant enzyme expression and their activities. Also, they showed lesser malondialdehyde content and electrolyte leakage compared to control tobacco plants. Further, transgenic plants produced higher degree of pectin methyl esterification via decreasing pectin methyl esterase (PME) activity in roots and leaves under unstressed and salt stressed conditions. The effect of flavan-3-ols on pectin methyl esterification under salt stressed conditions was further validated through in vitro experiments in which non-transgenic (wild) tobacco seedlings were exposed to salt stress in presence of flavan-3-ols, epicatechin and epigallocatechin. The in vitro exposed seedlings showed similar trend of increase in pectin methyl esterification through decreasing PME activity as observed in CsF3H transgenic lines. Taken together, overexpression of CsF3H provided tolerance to salt stress and fungus A. solani infection to transgenic tobacco through improved antioxidant system and enhanced pectin methyl esterification.read more
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Journal ArticleDOI
Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling
Luo Ping,Yuxiao Shen,Shuangxia Jin,Shasha Huang,Xu Cheng,Zhen Wang,Penghui Li,Jian Zhao,Manzhu Bao,Ning Guogui +9 more
TL;DR: In isolation of RrANR, the homologous gene from rose, and NtABF, an ABA-response related transcription factor gene from tobacco, indicate that overexpression of RwANR results in an increase in plant tolerance to oxidative stress via increased scavenging of ROS and modulation of the ABA signaling pathway.
Journal ArticleDOI
Molecular characterization of flavanone 3-hydroxylase gene and flavonoid accumulation in two chemotyped safflower lines in response to methyl jasmonate stimulation.
TL;DR: The results showed that the high expression of CtF3H in quinochalcone-type safflower line was associated with the accumulation of both quinchalcones and flavonols, whereas its low expression did not affect the increased accumulation of glycosylated derivatives.
Journal ArticleDOI
Overexpression of a tomato flavanone 3-hydroxylase-like protein gene improves chilling tolerance in tobacco.
TL;DR: Results suggested that SlF3HL stimulated flavonoid biosynthesis in response to chilling stress, which showed not only faster seed germination, better survival and growth, but also lower malondialdehyde accumulation, relative electrical conductivity and H2O2 and O2(·-) levels compared with WT plants.
Journal ArticleDOI
Flavanone-3-Hydroxylase Plays an Important Role in the Biosynthesis of Spruce Phenolic Defenses Against Bark Beetles and Their Fungal Associates.
Almuth Hammerbacher,Dineshkumar Kandasamy,Chhana Ullah,Axel Schmidt,Louwrance P. Wright,Jonathan Gershenzon +5 more
TL;DR: A significant increase in dihydroflavonol and flavan-3-ol content is observed after inoculating Norway spruce with the bark beetle vectored fungus and F3H plays a key role in the biosynthesis of defense compounds in NorwaySpruce that act against the bark beetles-fungus complex.
Journal ArticleDOI
Functional Analysis of Two Flavanone-3-Hydroxylase Genes from Camellia sinensis: A Critical Role in Flavonoid Accumulation.
Yahui Han,Keyi Huang,Yajun Liu,Tianming Jiao,Guoliang Ma,Yumei Qian,Peiqiang Wang,Xinlong Dai,Liping Gao,Tao Xia +9 more
TL;DR: Cloned CsF3Hs played critical roles in flavonoid biosynthesis in tea plants and showed that they were highly expressed in the tender leaves of tea plants.
References
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