Parahydrogen‐Induced Polarization of Amino Acids
TLDR
An overview of the current literature and developments in the hyperpolarization of amino acids and peptides can be found in this paper, where the authors provide an overview of some of the most relevant papers.Abstract:
Nuclear magnetic resonance (NMR) has become a universal method for biochemical and biomedical studies, including metabolomics, proteomics, and magnetic resonance imaging (MRI). By increasing the signal of selected molecules, the hyperpolarization of nuclear spin has expanded the reach of NMR and MRI even further (e.g. hyperpolarized solid-state NMR and metabolic imaging in vivo). Parahydrogen (pH2 ) offers a fast and cost-efficient way to achieve hyperpolarization, and the last decade has seen extensive advances, including the synthesis of new tracers, catalysts, and transfer methods. The portfolio of hyperpolarized molecules now includes amino acids, which are of great interest for many applications. Here, we provide an overview of the current literature and developments in the hyperpolarization of amino acids and peptides.read more
Citations
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Journal ArticleDOI
Parahydrogen Hyperpolarization Allows Direct NMR Detection of α-Amino Acids in Complex (Bio)mixtures.
TL;DR: In this article, an approach to non-hydrogenative parahydrogen hyperpolarization (nhPHIP) for unmodified α-amino acids that allows their detection and quantification in complex mixtures at sub-micromolar concentrations was presented.
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Recent advances in the application of parahydrogen in catalysis and biochemistry
Gerd Buntkowsky,Franziska Theiß,Jonas Lins,Yuliya A. Miloslavina,Laura Wienands,Alexey S. Kiryutin,Alexandra V. Yurkovskaya +6 more
TL;DR: Parahydrogen induced polarization (PHIP) is an efficient and cost-effective hyperpolarization method, which has widespread applications in Chemistry, Physics, Biochemistry, Biophysics, and Medical Imaging as mentioned in this paper .
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Advancing homogeneous catalysis for parahydrogen-derived hyperpolarisation and its NMR applications
TL;DR: Parahydrogen-induced polarisation (PHIP) is a nuclear spin hyperpolarization technique employed to enhance NMR signals for a wide range of molecules as mentioned in this paper .
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Optimizing the Reaction Conditions for the Formation of Fumarate via Trans-Hydrogenation
TL;DR: In this paper, the authors investigate the solution preparation protocols and the reaction conditions on the rate and yield of fumarate formation, and report conditions to reproducibly yield over 100mM fumarate on a short timescale, and discuss aspects of the protocol that hinder the formation of fumarates.
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Synthesis of 13C and 2H Labeled Vinyl Pyruvate and Hyperpolarization of Pyruvate
Arne Brahms,Andrey N. Pravdivtsev,Tim Stamp,Frowin Ellermann,Frank D. Sönnichsen,Jan-Bernd Hövener,Rainer Herges +6 more
TL;DR: An efficient new method for the preparation of vinyl esters, including 13 C labeled, fully deuterated vinyl pyruvate using a palladium catalyzed procedure is presented.
References
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Parahydrogen and synthesis allow dramatically enhanced nuclear alignment
TL;DR: The PASADENA effect as mentioned in this paper is a method for transient high-sensitivity proton spin-labelling by molecular addition of dihydrogen, which can be converted to magnetization observable by NMR.
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Reversible Interactions with para-Hydrogen Enhance NMR Sensitivity by Polarization Transfer
Ralph W. Adams,Juan A. Aguilar,Kevin D. Atkinson,Michael J. Cowley,Paul I. P. Elliott,Simon B. Duckett,Gary G. R. Green,Iman G. Khazal,Joaquín López-Serrano,David C. Williamson +9 more
TL;DR: It is shown here that a metal complex can facilitate the reversible interaction of para-hydrogen with a suitable organic substrate such that up to an 800-fold increase in proton, carbon, and nitrogen signal strengths are seen for the substrate without its hydrogenation.
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Transformation of symmetrization order to nuclear-spin magnetization by chemical reaction and nuclear magnetic resonance.
TL;DR: A method of obtaining very large nuclear-spin polarizations and a means of extending the resultant sensitivity enhancement to other spins is proposed in which the transfer of order occurs through population differences not associated with magnetization.
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The acquisition of multidimensional NMR spectra within a single scan
TL;DR: A scheme enabling the complete sampling of multidimensional NMR domains within a single continuous acquisition is introduced and exemplified, which could enable the characterization of transient events such as proteins folding, 2D NMR experiments on samples being chromatographed, and the incorporation of spectroscopic 2D sequences into in vivo imaging investigations.