Open AccessJournal Article
Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume.
Rahayu Utami,Nurhasniza Khalid,Mohd Aspollah Sukari,Mawardi Rahmani,Ahmad Bustaman Abdul,Dachriyanus +5 more
TLDR
In this investigation, triterpenoids friedelin, epifriedelanol and β-sitosterol were isolated from Elaeocarpus floribundus leaves and stem bark and promising antioxidant activity was found.Abstract:
Elaeocarpus floribundus is higher plant that has been used as traditional medicine for treating several diseases. There is no previous report on phytochemicals and bioactivity studies of this species. In this investigation, triterpenoids friedelin, epifriedelanol and β-sitosterol were isolated from its leaves and stem bark. Determination of total phenolic content of methanolic extract of leaves and stem bark was carried out using Folin-Ciocalteu reagent. All extracts and isolated compounds were subjected to screening of antioxidant activity using DPPH free radical scavenging method and cytotoxic activities by MTT assay towards human T4 lymphoblastoid (CEM-SS) and human cervical (HeLa) cancer cells. In the total phenolic content determination, methanolic extract of leaves gave higher value of 503.08±16.71 mg GAE/g DW than stem bark with value of 161.5±24.81 mg GAE/g DW. Polar extracts of leaves and stem bark possessed promising antioxidant activity with methanol extract of stem bark exhibited strongest activity with IC50 value of 7.36±0.01 μg/ml. In the cytotoxic activity assay, only chloroform extract of leaves showed significant activity with IC50 value of 25.6±0.06 μg/ml against CEM-SS cancer cell, while friedelin and epifriedelanol were found to be active against the two cancer cells with IC50 values ranging from 3.54 to 11.45 μg/ml.read more
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Cannabis Roots: A Traditional Therapy with Future Potential for Treating Inflammation and Pain.
TL;DR: The roots of the cannabis plant have a long history of medical use stretching back millennia, but the therapeutic potential has been largely ignored in modern times, with only a few studies examining the composition of cannabis root and its medical potential.
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Anticancer and antioxidant effects of extracts from different parts of indigo plant
Buk-Gu Heo,Yun-Jum Park,Yong-Seo Park,Jong-Hyang Bae,Ja-Yong Cho,Kun Park,Zenon Jastrzebski,Shela Gorinstein +7 more
TL;DR: Investigation in vitro revealed that the extracts of the indigo plant were found to be effective in suppressing the proliferation of cancer cells, although the results varied depending on the different parts of P. tinctorium Lour.
Journal ArticleDOI
Cork: Current Technological Developments and Future Perspectives for this Natural, Renewable, and Sustainable Material
TL;DR: In this article, a review of the use of cork as a potential source of chemicals for alternative applications is presented, focusing on suberin, extractives fraction, and use of the cork in nontraditional applications.
Journal ArticleDOI
Traditional knowledge of wild edible plants with special emphasis on medicinal uses in Southern Shan State, Myanmar.
TL;DR: The age class of 30–39 years was more familiar with the environments where they could collect WEPs and had more knowledge of W EPs than did the older groups, and the use of Elaeocarpus floribundus as an edible oil is a very interesting tradition.
Journal ArticleDOI
In vitro cytotoxic potential of friedelin in human MCF-7 breast cancer cell: Regulate early expression of Cdkn2a and pRb1, neutralize mdm2-p53 amalgamation and functional stabilization of p53.
TL;DR: Friedelin effectively inhibit breast cancer MCF-7 cell growth, it was associated with early expression of Cdkn1a, pRb2 and activation of p53 and caspases.
References
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Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal Article
Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents
TL;DR: In this paper, the use of Folin-Ciocalteu reagent rather than the FolinDenis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period was investigated.
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Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products
TL;DR: In this article, the antioxidant activity and total phenolics of 28 plant products, including sunflower seeds, flaxseeds, wheat germ, buckwheat, and several fruits, vegetables, and medicinal plants were determined.
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Feasibility of Drug Screening with Panels of Human Tumor Cell Lines Using a Microculture Tetrazolium Assay
Michael C. Alley,Dominic A. Scudiero,Anne Monks,Miriam L. Hursey,Maciej Czerwinski,Donald L. Fine,B. J. Abbott,Joseph G. Mayo,Robert H. Shoemaker,Michael R. Boyd +9 more
TL;DR: Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Journal ArticleDOI
13C NMR spectra of pentacyclic triterpenoids--a compilation and some salient features
Shashi B. Mahato,Asish P. Kundu +1 more
TL;DR: In this paper, a compilation of the 13C NMR data of a selected variety of naturally occurring pentacyclic triterpenoids, arranged skeletonwise, is provided.