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Journal ArticleDOI

Photosynthesis by isolated chloroplasts I. Diffusion of labeled photosynthetic intermediates between isolated chloroplasts and suspending medium

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TLDR
An experiment in which changes in the level of ATP were observed on addition of CO 2 shows that ATP and ADP can apparently re-enter the chloroplasts and be used metabolically.
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This article is published in Biochimica et Biophysica Acta.The article was published on 1968-01-15. It has received 123 citations till now. The article focuses on the topics: Photosynthesis & Chloroplast.

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Citations
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Journal ArticleDOI

The role of pH in the regulation of carbon fixation in the chloroplast stroma. Studies on CO2 fixation in the light and dark.

TL;DR: It is shown that intact chloroplasts are able to fix CO2 in the dark, yielding 3-phosphoglycerate, and demonstrates that CO2 fixation in thedark is not possible, unless the pH in the medium is artificially raised to pH 8.8.
Journal ArticleDOI

Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts.

TL;DR: The uptake of 3-phosphoglycerate and malate is regarded as a transport across the inner membrane into the stroma space, different from the anion permeability of the thylakoid membrane studied earlier.
Journal ArticleDOI

Reversible inhibition of the calvin cycle and activation of oxidative pentose phosphate cycle in isolated intact chloroplasts by hydrogen peroxide

TL;DR: Results indicate that hydrogen peroxide interacts with reduced sulfhydryl groups which are involved in the light activation of enzymes of the Calvin cycle at the site of fructose- and sedoheptulose bisphophatase, of phosphoribulokinase, as well as in light-inactivation of oxidative pentose phosphate cycle atThe site of glucose-6-phosphate dehydrogenase.
Book ChapterDOI

Physiology and Metabolism of Phosphate and Its Compounds

TL;DR: The distribution of phosphorus in the world, unlike that of all other elements but carbon, is dominated by the present and past activities of living organisms as mentioned in this paper, and most of the phosphate used by man has been formed either as guano and its end-product, phosphatized coral, or as sedimentary deposits laid down under marine conditions in a combination of biological and physicochemical processes.
Journal ArticleDOI

Ferredoxin-activated Fructose Diphosphatase of Spinach Chloroplasts: RESOLUTION OF THE SYSTEM, PROPERTIES OF THE ALKALINE FRUCTOSE DIPHOSPHATASE COMPONENT, AND PHYSIOLOGICAL SIGNIFICANCE OF THE FERREDOXIN-LINKED ACTIVATION

TL;DR: The activity of chloroplast FDPase during photosynthesis appears to be controlled photochemically through reduced ferredoxin, and what, if any, physiological role can be ascribed to the activation of the enzyme by high concentrations of Mg++ and disulfide reagents, independently of reduced fer redoxin is not clear.
References
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ReportDOI

The Path of Carbon in Photosynthesis. XXI. The Cyclic Regenerationof Carbon Dioxide Acceptor

TL;DR: In this paper, the distribution of radiocarbon in these sugars, and other data, indicate that sedoheptulose phosphate and ribulose diphosphates are formed during photosynthesis from triose and hexose phosphates, the latter being synthesized by the reduction of 3-phosphoglyceric acid.
Journal ArticleDOI

Light‐Dark Transients in Levels of Intermediate Compounds during Photosynthesis in Air‐Adapted Chlorella

TL;DR: The labeling of intermediate compounds and photosynthetic cofactors by Chlorella pyrenoidosa in the presence of 32P-labeled phosphate and 14CO2 have heen investigated and large transient changes in the level of pyrophosphate between light and dark indicate that there may be an unstable cofactor which decomposes to give pyroph phosphate during or alter killing of the algal cells.
Journal ArticleDOI

Inhibition of photophosphorylation and photosynthetic carbon cycle reactions by fatty acids and esters.

TL;DR: Several key steps in the photosynthetic carbon reduction cycle may be mediated by enzymes in some organized system associated with the lamellae and photophosphorylation, resulting in decreased rates of conversion of ribulose-5-phosphate to ribulinose-1,5-diphosphate, of glucose-6-ph phosphate to polysaccharides and sucrose, and other reactions.
Journal ArticleDOI

Photosynthesis by isolated chloroplasts III. Light activation of the carboxylation reaction

TL;DR: It appears that the primary cause of decreased rate of fixation with time in these highly active isolated chloroplasts is loss of activity of the carboxylation enzyme, and the principal cause may be loss of the light activation.
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