Photosystem II Supercomplex Remodeling Serves as an Entry Mechanism for State Transitions in Arabidopsis
Lars Dietzel,Katharina Bräutigam,Sebastian Steiner,Kristin Schüffler,Bernard Lepetit,Bernhard Grimm,Mark Aurel Schöttler,Thomas Pfannschmidt +7 more
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TLDR
This work shows in Arabidopsis thaliana that PSII supercomplex remodeling precedes and facilitates state transitions, and indicates that PSii supercomplex generation is independently regulated and does not require light-harvesting complex phosphorylation and movement.Abstract:
Within dense plant populations, strong light quality gradients cause unbalanced excitation of the two photosystems resulting in reduced photosynthetic efficiency. Plants redirect such imbalances by structural rearrangements of the photosynthetic apparatus via state transitions and photosystem stoichiometry adjustments. However, less is known about the function of photosystem II (PSII) supercomplexes in this context. Here, we show in Arabidopsis thaliana that PSII supercomplex remodeling precedes and facilitates state transitions. Intriguingly, the remodeling occurs in the short term, paralleling state transitions, but is also present in a state transition–deficient mutant, indicating that PSII supercomplex generation is independently regulated and does not require light-harvesting complex phosphorylation and movement. Instead, PSII supercomplex remodeling involves reversible phosphorylation of PSII core subunits (preferentially of CP43) and requires the luminal PSII subunit Psb27 for general formation and structural stabilization. Arabidopsis knockout mutants lacking Psb27 display highly accelerated state transitions, indicating that release of PSII supercomplexes is required for phosphorylation and subsequent movement of the antenna. Downregulation of PSII supercomplex number by physiological light treatments also results in acceleration of state transitions confirming the genetic analyses. Thus, supercomplex remodeling is a prerequisite and an important kinetic determinant of state transitions.read more
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Journal ArticleDOI
Photosystem II repair in plant chloroplasts--Regulation, assisting proteins and shared components with photosystem II biogenesis.
TL;DR: This review focuses on the current knowledge of regulation of the repair cycle of PSII in higher plant chloroplasts and the function of the number ofPSII auxiliary proteins involved both in the biogenesis and repair of PS II.
Journal ArticleDOI
Assembling and maintaining the Photosystem II complex in chloroplasts and cyanobacteria
TL;DR: Results show that assembly of PSII occurs in a step-wise fashion in defined regions of the membrane system, involves conserved auxiliary factors and is closely coupled to chlorophyll biosynthesis.
Journal ArticleDOI
Structure and dynamics of thylakoids in land plants
TL;DR: An update on the roles of lipids, proteins, and protein complexes, as well as possible trafficking mechanisms, during thylakoid biogenesis and the de-etiolation process complements this review.
Journal ArticleDOI
Functional Analyses of the Plant Photosystem I–Light-Harvesting Complex II Supercomplex Reveal That Light-Harvesting Complex II Loosely Bound to Photosystem II Is a Very Efficient Antenna for Photosystem I in State II
Pierre Galka,Stefano Santabarbara,Thi Thu Khuong Khuong,Thi Thu Khuong Khuong,Thi Thu Khuong Khuong,Hervé Degand,Pierre Morsomme,Robert C. Jennings,Egbert J. Boekema,Stefano Caffarri,Stefano Caffarri,Stefano Caffarri +11 more
TL;DR: Fluorescence analyses indicate that excitation energy migration from mobile LHCII to PSI is rapid and efficient, and the quantum yield of photochemical conversion of PSI-LHCII is substantially unaffected with respect to PSi, despite a sizable increase of the antenna size.
Journal ArticleDOI
The light-harvesting chlorophyll a/b binding proteins Lhcb1 and Lhcb2 play complementary roles during state transitions in Arabidopsis.
Malgorzata Pietrzykowska,Marjaana Suorsa,Dmitry A. Semchonok,Mikko Tikkanen,Egbert J. Boekema,Eva-Mari Aro,Stefan Jansson +6 more
TL;DR: The detailed architecture of the extant seed plant light-harvesting antenna can now be dated back to a time after the divergence of the bryophyte and spermatophyte lineages, but before the split of the angiosperm and gymnosperm lineages more than 300 million years ago.
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