Journal ArticleDOI
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
TLDR
In this paper, the extinction coefficients for chlorophylls a and b in diethylether (Smith, J.H. and Benitez, A.V., eds.), used in this paper as primary standards, were verified by magnesium determination using atomic absorbance spectrophotometry.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1989-08-01. It has received 5326 citations till now. The article focuses on the topics: Chlorophyll & Chlorophyll a.read more
Citations
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Journal ArticleDOI
The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution*
TL;DR: In this paper, specific absorption coefficients for individual carotenoids and chlorophylls a and b, as well as the E 1% 1cm values for combined carotensoids, have been (re)estimated using 6 solvents (80 % acetone, chloroform, diethyl ether, dimethyl formamide and methanol) using two different types of spectrophotometer (0.1-0.5 nm and 1-4 nm band pass resolution).
Journal ArticleDOI
Atomic model of plant light-harvesting complex by electron crystallography
TL;DR: The structure of the light-harvesting chlorophyll a/b–protein complex, an integral membrane protein, has been determined at 3.4 Å resolution by electron crystallography of two-dimensional crystals.
Journal ArticleDOI
Integrated narrow-band vegetation indices for prediction of crop chlorophyll content for application to precision agriculture
D. Haboudane,John R. Miller,John R. Miller,Nicolas Tremblay,Pablo J. Zarco-Tejada,Louise Dextraze +5 more
TL;DR: In this paper, a combined modeling and indices-based approach is presented to predict the crop chlorophyll content from remote sensing data while minimizing LAI (vegetation parameter) influence and underlying soil background effects.
Journal ArticleDOI
The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b
TL;DR: How the ratios are increasingly and alarmingly low as the proportion of chlorophyll a increases is described, which is always low.
Journal ArticleDOI
Photosynthetic acclimation of plants to growth irradiance: the relative importance of specific leaf area and nitrogen partitioning in maximizing carbon gain
John R. Evans,Hendrik Poorter +1 more
TL;DR: Changes in specific leaf area (SLA, projected leaf area per unit leaf dry mass) and nitrogen partitioning between proteins within leaves occur during the acclimation of plants to their growth irradiance, and the relative importance of both of these changes in maximizing carbon gain is quantified.
References
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Journal ArticleDOI
Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
TL;DR: Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
Book ChapterDOI
Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes
TL;DR: In this article, the spectral characteristics and absorption coefficients of chlorophylls, pheophytins, and carotenoids were analyzed using a two-beam spectrophotometer.
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New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton
S.W. Jeffrey,G.F. Humphrey +1 more
TL;DR: New equations are presented for spectrophotometric determination of chlorophylls, based on revised extinction coefficients of chloropylls a, b, c1 and c2, which may be used for determining chlorophyLLs a and b in higher plants and green algae.
Journal ArticleDOI
Absorption of light by chlorophyll solutions
TL;DR: This paper deals with the estimation of chlorophyll in plant extracts by application of absorption coefficients of the isolated solid chlorophylla components, and the question of artifacts is automatically clarified.