Journal ArticleDOI
Phylogeography and molecular epidemiology of Papaya ringspot virus
X.A. Olarte Castillo,Gustavo Fermin,Javier F. Tabima,Y. Rojas,Paula F. Tennant,Marc Fuchs,Roberto Sierra,Adriana Bernal,Silvia Restrepo +8 more
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TLDR
The results confirm previous estimates on the origin of PRSV around 400 years ago and suggest distinct dispersion events from the Indian Peninsula to the rest of Asia, via Thailand, and subsequently to the Americas.About:
This article is published in Virus Research.The article was published on 2011-08-01. It has received 60 citations till now. The article focuses on the topics: Papaya ringspot virus & Carica.read more
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Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.
TL;DR: It is suggested that the natural selection against large insertion/deletion is so weak that a large amount of variation is maintained in a population.
Journal ArticleDOI
Use of RNAi technology to develop a PRSV-resistant transgenic papaya
Jia Ruizong,Hui Zhao,Jing Huang,Jing Huang,Hua Kong,Yuliang Zhang,Guo Jingyuan,Qixing Huang,Yunling Guo,Qing Wei,Jiao Zuo,Yun J. Zhu,Ming Peng,Anping Guo +13 more
TL;DR: This study designed a comprehensive RNAi strategy targeting the conserved domain of the PRSV CP gene to develop a broader-spectrum transgenic resistance to the Hainan PRV isolates, and used an optimized particle-bombardment transformation system to produce RNAi-CP-transgenic papaya lines.
Journal ArticleDOI
Plant virus ecology and epidemiology: historical perspectives, recent progress and future prospects
TL;DR: There is an urgent need to understand how viruses move in both directions between the two and be able to anticipate these kinds of events, and to obtain an understanding of, and ability to foresee, such events will require a major research effort into the future.
Journal ArticleDOI
The Potyviruses: An Evolutionary Synthesis Is Emerging.
TL;DR: The likely evolutionary pathways that have resulted in the observed similarities and differences of the extant molecules, biology, distribution, etc. of the potyvirids and, especially, its largest genus, the Potyviruses are outlined.
Journal ArticleDOI
Evolutionary history and variation in host range of three Stagonosporopsis species causing gummy stem blight of cucurbits
TL;DR: Using a multilocus sequencing approach, it is discovered that gummy stem blight of cucurbits is caused by three genetically distinct species: Stagonosporopsis cucurbitacearum, S. citrulli and S. caricae, which had previously been considered only a pathogen of papaya.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
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A new look at the statistical model identification
TL;DR: In this article, a new estimate minimum information theoretical criterion estimate (MAICE) is introduced for the purpose of statistical identification, which is free from the ambiguities inherent in the application of conventional hypothesis testing procedure.
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
Book
Molecular cloning : a laboratory manual
Joseph Sambrook,David W. Russell +1 more
TL;DR: The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
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MODELTEST: testing the model of DNA substitution.
David Posada,Keith A. Crandall +1 more
TL;DR: The program MODELTEST uses log likelihood scores to establish the model of DNA evolution that best fits the data.