Open AccessJournal Article
Physicochemical and phytochemical standardization with HPTLC fingerprinting of Nigella sativa L. seeds.
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TLDR
The physicochemical and phytochemical standards along with HPTLC fingerprint profile established as an outcome of this research may be utilized as substantial data for identification, purification and standardization of Nigella sativa seeds.Abstract:
This study was designed to perform the physicochemical and phytochemical standardization with HPTLC fingerprinting of Nigella sativa seeds in order to establish the standard pharmacognostical parameters of this miracle herb. Different parameters like extractive values; total ash value, acid insoluble ash value and water soluble ash value, moisture content, loss on drying, pH values of Nigella sativa seeds were performed. Preliminary phytochemical screening was done to detect different phytoconstituents by using the Harborne's phytochemical methods. Quantification of phenolic and flavonoid contents, determination of pesticides residues, aflatoxin and heavy metals were also carried out. HPTLC fingerprinting of methanolic extract was performed using CAMAG-HPTLC system connected with win CAT software. Preliminary phytochemical screening of the extracts in different solvent revealed the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, lipids, sterols and tannins. Total flavonoid and phenolic contents in methanolic extract was found to be 1.4 mg/gm and 9.8 mg/gm extract respectively. Concentrations of heavy metals were found within acceptable limits. Pesticides residues and aflatoxins were not detected. The physicochemical and phytochemical standards along with HPTLC fingerprint profile established as an outcome of this research may be utilized as substantial data for identification, purification and standardization of Nigella sativa seeds.read more
Citations
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Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits
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TL;DR: In this paper, the results of phytochemicals testing indicated the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, lipids, sterols and tannins in various solvent extracts.
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Female infertility and herbal medicine: An overview of the new findings.
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Phytochemical Extraction and Screening of Bio Active Compounds from Black Cumin (Nigella Sativa) Seeds Extract
TL;DR: It can scientifically conclude that the cumin seeds extract could be seen as an increasingly valuable reservoir of bioactive compounds of substantial medicinal merit.
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Ameliorative Effect of Camel's Milk and Nigella Sativa Oil against Thioacetamide-induced Hepatorenal Damage in Rats.
Aftab Ahmad,Fahad A. Al-Abbasi,Saida Sadath,Soad Shaker Ali,Mohammed F. Abuzinadah,Hani A. Alhadrami,Anwar Ali Mohammad Alghamdi,Ali H Aseeri,Shah Alam Khan,Asif Husain +9 more
TL;DR: Treatment of rats with CM, NSO, and CM plus NSO combination and Jigreen significantly reversed the damage and brought down the serum biochemical parameters and lipid profile toward the normal levels in serum.
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Quality variation and standardization of black pepper (Piper nigrum): A comparative geographical evaluation based on instrumental and metabolomics analysis
Rizwan Ahmad,Niyaz Ahmad,Mohd Amir,Fatema Aljishi,Marwah Hussain Alamer,Heba Radhi Al-Shaban,Zainab Alawi Alsadah,Bayan Mohammed Alsultan,Noor Ali Aldawood,Shahanas Chathoth,Sarah Ameen Almofty +10 more
TL;DR: The samples were properly standardized and effectively differentiated in terms of quality and biological activities using fast and reliable tools, however it certainly does not mean that a particular geographical region is more better or productive in termsof herbal products.
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