Journal ArticleDOI
Plant Genome Size Estimation by Flow Cytometry: Inter-laboratory Comparison
Jaroslav Doležel,Johann Greilhuber,Sergio Lucretti,Armin Meister,Martin A. Lysak,Luca Nardi,R Obermayer +6 more
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TLDR
The results obtained in this study demonstrate that flow cytometry with DNA intercalators is a reliable method for estimation of nuclear genome size in plants, and confirmed an urgent need for an agreement on standards.About:
This article is published in Annals of Botany.The article was published on 1998-12-01. It has received 583 citations till now. The article focuses on the topics: Feulgen stain.read more
Citations
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A physical, genetic and functional sequence assembly of the barley genome
Klaus F. X. Mayer,Robbie Waugh,Peter Langridge,Timothy J. Close,Roger P. Wise,Andreas Graner,Takashi Matsumoto,Kazuhiro Sato,Alan H. Schulman,Ruvini Ariyadasa,Daniela Schulte,Naser Poursarebani,Ruonan Zhou,Burkhard Steuernagel,Martin Mascher,Uwe Scholz,Bu-Jun Shi,Kavitha Madishetty,Jan T. Svensson,Prasanna R. Bhat,Matthew J. Moscou,Josh Resnik,Gary J. Muehlbauer,Pete E. Hedley,Hui Liu,Jenny Morris,Zeev Frenkel,Avraham Korol,Hélène Bergès,Stefan Taudien,Marius Felder,Marco Groth,Matthias Platzer,Axel Himmelbach,Stefano Lonardi,Denisa Duma,Matthew Alpert,Francesa Cordero,Francesa Cordero,Marco Beccuti,Gianfranco Ciardo,Yaqin Ma,Steve Wanamaker,Federica Cattonaro,Vera Vendramin,Simone Scalabrin,Slobodanka Radovic,Rod A. Wing,Michele Morgante,Thomas Nussbaumer,Heidrun Gundlach,Mihaela Martis,Jesse Poland,Matthias Pfeifer,Cédric Moisy,Jaakko Tanskanen,Andrea Zuccolo,Manuel Spannagl,Joanne Russell,Arnis Druka,David Marshall,Micha Bayer,David Swarbreck,Dharanya Sampath,Sarah Ayling,Melanie Febrer,Mario Caccamo,Tsuyoshi Tanaka,Steve Wannamaker,Thomas Schmutzer,John W. S. Brown,John W. S. Brown,Geoffrey B. Fincher,Nils Stein +73 more
TL;DR: An integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context and suggests that post-transcriptional processing forms an important regulatory layer.
Journal ArticleDOI
Estimation of nuclear DNA content in plants using flow cytometry.
TL;DR: Four protocols for sample preparation (suspensions of intact cell nuclei) and the analysis of nuclear DNA amounts using FCM are presented and the most frequent problems encountered with plant material such as the interference of secondary metabolites are described.
Journal ArticleDOI
Nuclear DNA content and genome size of trout and human.
TL;DR: Because trout and human nuclei are often used as internal reference standards to determine genome size in animals and plants, the results published by Thomas et al. (1) need correction to avoid serious mistakes.
Journal ArticleDOI
Plant DNA flow cytometry and estimation of nuclear genome size.
Jaroslav Doležel,Jan Bartoš +1 more
TL;DR: Current procedures for estimation of absolute DNA amounts in plants using flow cytometry are reviewed, with special emphasis on preparation of nuclei suspensions, stoichiometric DNA staining and the use of DNA reference standards.
Journal ArticleDOI
Brachypodium distachyon. A new model system for functional genomics in grasses.
John Draper,Luis A. J. Mur,Glyn Jenkins,Gadab C. Ghosh-Biswas,Pauline Bablak,Robert Hasterok,Andrew P. M. Routledge +6 more
TL;DR: A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of "core pooid" genera that contain the majority of important temperate cereals and forage grasses.
References
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Journal ArticleDOI
Rapid flow cytometric analysis of the cell cycle in intact plant tissues.
David W. Galbraith,Kristi R. Harkins,Joyce M. Maddox,Nicola M. Ayres,Dharam P. Sharma,Ebrahim Firoozabady +5 more
TL;DR: The amount of nuclear DNA in the homogenates of monocotyledonous and dicotylingonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
Michael D. Bennett,J. B. Smith +1 more
TL;DR: This paper lists absolute nuclear DNA amounts for 753 angiosperm species, primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper lists absolute nuclear DNA amounts for 753 angiosperm species, and the dats were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users.
Journal ArticleDOI
Nuclear DNA Amounts in Angiosperms
TL;DR: This paper contains a supplementary list of absolute DNA values, including estimates for 240 angiosperm species not listed by Bennett & Smith in 1976, as well as additional estimates for 41 species already listed by them.
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Nuclear DNA Content and Minimum Generation Time in Herbaceous Plants
TL;DR: It is shown that the mean cell cycle time and the mean meiotic duration in annual species is significantly shorter than in perennial species, and that satellite DNA is significant in its nucleotypic effects on developmental processes.