Polyacrylamide Slab Gel Electrophoresis of Soluble Proteins for Studies of Bacterial Floras
TLDR
A polyacrylamide slab gel electrophoresis procedure was used to compare cellular proteins from bacterial isolates of gingival crevice floras and provided additional verification of the identity of strains characterized by conventional phenotypic tests.Abstract:
A polyacrylamide slab gel electrophoresis procedure was used to compare cellular proteins from bacterial isolates of gingival crevice floras. Isolates with identical protein patterns consistently were shown to be members of the same species. When used to screen isolates, the procedure reduced total analytical time and expense without sacrificing accuracy, and it provided additional verification of the identity of strains characterized by conventional phenotypic tests. Imagesread more
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Book ChapterDOI
Phenotypic Characterization and the Principles of Comparative Systematics
TL;DR: The methods described in this chapter are based primarily on methods developed for the characterization and identification of organisms which have usually been isolated on nutrient-rich media.
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Intestinal floras of populations that have a high risk of colon cancer.
W. E. C. Moore,L. V. H. Moore +1 more
TL;DR: Fecal floras of polyp patients, Japanese-Hawaiians, North American Caucasians, rural native Japanese, and rural native Africans were compared, and total concentrations of Bacteroides species and, surprisingly, Bifidobacterium species were generally positively associated with increased risk of colon cancer.
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Bacteriology of severe periodontitis in young adult humans.
TL;DR: Of 190 bacterial species, subspecies, or serotypes detected among 2,723 isolates, 11 species exceeded 1% of the subgingival flora and were most closely associated with the diseased sulci and were also sufficiently frequent to be suspect agents of tissue destruction.
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Comparative bacteriology of juvenile periodontitis.
TL;DR: Of 357 bacterial taxa among over 18,000 isolates, 54 non-treponemal species, 2 treponemals species, and mycoplasma were most associated with diseased periodontal sulci and constituted over half of the cultivable flora of diseased sites.
Journal ArticleDOI
Bacteriology of moderate (chronic) periodontitis in mature adult humans.
TL;DR: The sulcus floras of moderate periodontitis and severeperiodontitis shared many of their predominant bacterial species, but there were differences in the relative proportions of some of these species.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
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Journal ArticleDOI
Disc electrophoresis-i background and theory*
TL;DR: Some mechanisms that provide a rationale for the resolution afforded by zone electrophoresis in many gels will be detailed; the theory of some new modifications of zone electophoresis that have been designed to take maximum advantage of these mechanisms will be developed.