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Journal ArticleDOI

Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage

TLDR
A controlled anaerobic fermentation of beet silage, only initially inoculated with manure, was monitored by the amplified "ribosomal DNA" restriction analysis, finding that species distribution patterns for Firmicutes, Bacteroidetes, Synergistetes and Thermotogae were not clearly linked to process parameters.
About
This article is published in Bioresource Technology.The article was published on 2011-05-01. It has received 96 citations till now. The article focuses on the topics: Firmicutes & Anaerobic digestion.

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Journal ArticleDOI

Biogas Upgrading via Hydrogenotrophic Methanogenesis in Two-Stage Continuous Stirred Tank Reactors at Mesophilic and Thermophilic Conditions

TL;DR: The increase of hydrogenotrophic methanogens and syntrophic Desulfovibrio and the decrease of aceticlastic methanogen indicate a H2-mediated shift toward the hydrogenot microscopic pathway enhancing biogas upgrading.
Journal ArticleDOI

Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy

TL;DR: This study dissects the bioma involved in anaerobic digestion by means of high throughput Illumina sequencing, disclosing nearly one million genes and extracting 106 microbial genomes by a novel strategy combining two binning processes, establishing a reference catalog of biogas microbial genomes that will greatly simplify future genomic studies.
Journal ArticleDOI

Microbial community structures and functions of wastewater treatment systems in plateau and cold regions.

TL;DR: Investigation of microbial communities of plateau WWTPs showed that microbial diversities and richness were negatively associated with the altitude and positively to the water temperature to a certain extent, and functional genera and enzymes showed that there was no significant difference in abundances of organic degrading bacteria.
Journal ArticleDOI

Identification of syntrophic acetate-oxidizing bacteria in anaerobic digesters by combined protein-based stable isotope probing and metagenomics.

TL;DR: This study is the first study applying protein-SIP for analysis of complex biogas samples, a promising method for identifying key microorganisms utilizing specific pathways and revealed that Methanosarcina and Methanoculleus were actively involved in acetate turnover, as were five subspecies of Clostridia.
References
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16S/23S rRNA sequencing

D. J. Lane
Book

Nucleic acid techniques in bacterial systematics

TL;DR: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleics acid probes DNA fingerprinting from macromolecules to trees.
Journal ArticleDOI

Energy production from biomass (Part 1): Overview of biomass.

TL;DR: The potential of a restored landfill site to act as a biomass source, providing fuel to supplement landfill gas-fuelled power stations, is examined, together with a comparison of the economics of power production from purpose-grown biomass versus waste-biomass.
Journal ArticleDOI

Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity

TL;DR: The authors' perspective on microbial diversity has improved enormously over the past few decades in large part due to molecular phylogenetic studies that objectively relate organisms.
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