Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers
Raveendran Sindhu,Balakrishnan Ammu,Parameswaran Binod,Sreelatha K. Deepthi,K. B. Ramachandran,Carlos Ricardo Soccol,Ashok Pandey +6 more
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In this paper, the authors studied the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source.Abstract:
The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, 1H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC.read more
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Electro-Fermentation – Merging Electrochemistry with Fermentation in Industrial Applications
Andrea Schievano,Tommy Pepè Sciarria,Karolien Vanbroekhoven,Heleen De Wever,Sebastià Puig,Stephen Andersen,Korneel Rabaey,Deepak Pant +7 more
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References
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Journal ArticleDOI
Polymer blends and composites from renewable resources
Long Yu,Katherine Dean,Lin Li +2 more
TL;DR: A review of polymer blends and composites from renewable resources can be found in this article, where the progress of blends from three kinds of polymers from renewable sources (i.e., natural polymers such as starch, protein and cellulose), synthetic polymers, such as polylactic acid and polyhydroxybutyrate, are described with an emphasis on potential applications.
Journal ArticleDOI
High cell-density culture of Escherichia coli
TL;DR: The problems encountered in HCDC of E. coli are reviewed, various solutions are discussed, and feeding strategies for HCDC are described.
Journal ArticleDOI
ASSAY OF POLY-β-HYDROXYBUTYRIC ACID
John H. Law,Ralph A. Slepecky +1 more
TL;DR: This method has been used to follow the production of poly-β-hydroxybutyric acid by Bacillus megaterium strain KM and permits an accurate determination of this material in quantities down to 5 μg.
Journal ArticleDOI
A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds.
Patricia Spiekermann,Bernd H. A. Rehm,Rainer Kalscheuer,Dirk Baumeister,Alexander Steinbüchel +4 more
TL;DR: The oxazine dye Nile blue A and its fluorescent oxazone form, Nile red, were used to develop a simple and highly sensitive staining method to detect poly(3-hydroxybutyric acid) and other polyhydroxyalkanoic acids (PHAs) directly in growing bacterial colonies.
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Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate.
A G Ostle,John G. Holt +1 more
TL;DR: Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.