Journal ArticleDOI
Production of Alkaline Protease by Bacillus thuringiensis H 14 in Aqueous Two‐Phase Systems
Srinivas Hotha,R.M. Banik +1 more
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In this article, Bacillus thuringiensis H 14 was studied in aqueous two-phase systems composed of PEG X (X = 9000, 6000, 4000) and potassium phosphate.Abstract:
Alkaline protease production by Bacillus thuringiensis H 14 was studied in aqueous two-phase systems composed of PEG X (X = 9000, 6000, 4000) and potassium phosphate. The top phase is continuous and rich in PEG while the bottom phase is dispersed and is rich in phosphate, microbial cells being mainly retained in the bottom phase. The alkaline protease produced during fermentation, partitioned into the upper phase (about 80%) and total protease produced were about 2·80 and 2·26 times higher than that of homogeneous fermentation when the fermentations were carried out in aqueous two-phase systems from the beginning and made after 45 h of inoculation respectively. © 1997 SCI.read more
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Journal ArticleDOI
Bacterial alkaline proteases: molecular approaches and industrial applications
TL;DR: The types and sources of proteases, protease yield-improvement methods, the use of new methods for developing novel proteases and applications of alkaline proteases in industrial sectors are discussed, with an overview on the use in the detergent industry.
Journal ArticleDOI
Microbial alkaline proteases: From a bioindustrial viewpoint
C. Ganesh Kumar,Hiroshi Takagi +1 more
TL;DR: In this paper, a review of the proteases that can resist extreme alkaline environments produced by a wide range of alkalophilic microorganisms is presented, and various nutritional and environmental parameters affecting the production of alkaline proteases are delineated.
Journal Article
A Review on Microbial Alkaline Proteases
TL;DR: The protcascs that can resist extreme alkaline environments produced by a wide range of ulk alophilic microorg anisms are described and different isoluion methods which enable the screening and selection of promising organisms 1'01' industrial production are discussed.
Journal ArticleDOI
Technological aspects of extractive fermentation using aqueous two-phase systems
TL;DR: This review will consider the basic principles involved and factors affecting extractive fermentation, technical aspects and technological developments like use of ligands and surfactants to improve separation, recent trends and future prospects of Extractive fermentation systems.
Journal ArticleDOI
Versatility of microbial proteases
N. Jisha Veloorvalappil,B. Smitha Robinson,Pradeep Selvanesan,Sreedevi Sasidharan,N. Unni Kizhakkepawothail,Sajith Sreedharan,Priji Prakasan,Sarath Josh Moolakkariyil,Benjamin Sailas +8 more
TL;DR: An overview on alkaline proteases, mainly of microbial sources in a handy module, and an overview on the applications of proteases in detergent, tannery, food, metal recovery and waste treatment industries is addressed briefly.