Journal ArticleDOI
Production of lycopene by metabolically engineered Pichia pastoris.
Reads0
Chats0
TLDR
The lycopene yield in this study is the highest yield in P. pastoris reported to date, which demonstrated the potential of P. Pastoris in lycopenes synthesis and as a candidate host organism for the synthesis of other high value-added terpenoids.Abstract:
Lycopene is a highly valued carotenoid with wide applications in various industries. The market demand for lycopene promotes research in metabolic engineering of heterologous hosts for lycopene. In...read more
Citations
More filters
Journal ArticleDOI
Advanced Strategies for Production of Natural Products in Yeast.
TL;DR: Current progress in engineering yeast cell factories for production of natural products, including terpenoids, alkaloids, and phenylpropanoids is summarized and advanced strategies in metabolic engineering at three different dimensions are discussed.
Journal ArticleDOI
Biotechnological production of lycopene by microorganisms.
TL;DR: This work presents perspectives concerning the microbial production of lycopene, especially trends to construct microbial cell factories for lycopenes production, and discusses the current challenges and prospects of using microorganisms to produce lycopenoids.
Journal ArticleDOI
Recent Advances on Biological Synthesis of Lycopene by Using Industrial Yeast
Yiwen Jing,Feng Guo,Shangjie Zhang,Weiliang Dong,Jie Zhou,Fengxue Xin,Wenming Zhang,Min Jiang +7 more
TL;DR: The lycopene molecule has been widely applied in the food and medical fields due to having antioxidant, anticancer, and anti-inflammatory properties.
Journal ArticleDOI
Enhanced CO2 capture for photosynthetic lycopene production in engineered Rhodopseudomonas palustris, a purple nonsulfur bacteria
TL;DR: Lycopene has been widely applied in the fields of food additives, drug and cosmetics due to its anti-oxidative, anti-cancer, and anti-inflammatory activities.
Journal ArticleDOI
Metabolic Engineering of Different Microbial Hosts for Lycopene Production.
TL;DR: In this review, strategies used to improve lycopene production in different microbial hosts and the advantages and disadvantages of each microbial host are summarized.
References
More filters
Journal ArticleDOI
Heterologous protein production using the Pichia pastoris expression system.
TL;DR: The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins and the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed.
Journal ArticleDOI
Continuous-flow synthesis of the anti-malaria drug artemisinin.
TL;DR: Combined with the production of artemisinic acid in engineered yeast, access to the muchneeded malaria drug is now possible by semi-synthesis rather than isolation from plants, ensuring a steady supply of art Artemisinin at greatly reduced cost.
Book ChapterDOI
Expression in the yeast Pichia pastoris.
TL;DR: Procedures to be found here include: strain construction by classical yeast genetics, the logic in selection of a vector and strain, preparation of electrocompetent yeast cells and transformation by electroporation, and the yeast colony western blot or Yeastern blot method for visualizing secreted proteins around yeast colonies.
Journal ArticleDOI
Lipid engineering combined with systematic metabolic engineering of Saccharomyces cerevisiae for high-yield production of lycopene
Tian Ma,Bin Shi,Ziling Ye,Xiaowei Li,Min Liu,Yun Chen,Jiang Xia,Jens Nielsen,Zixin Deng,Tiangang Liu +9 more
TL;DR: This study overexpressed key genes associated with fatty acid synthesis and TAG production, followed by modulation of TAG fatty acyl composition by overexpressing a fatty acid desaturase (OLE1) and deletion of Seipin (FLD1), which regulates lipid-droplet size to enable increased lycopene accumulation.
Journal ArticleDOI
Lycopene: Antioxidant and Biological Effects and its Bioavailability in the Human
Helmut Sies,Wilhelm Stahl +1 more
TL;DR: In this article, the major dietary sources of Iycopene for the human are tomatoes and tomato products, and the Protective effects of a IYcopene-containing tomato product are discussed.