Journal ArticleDOI
Properties of infectious ribonucleic acid from wheat streak mosaic virus.
Myron K. Brakke,Nancy Van Pelt +1 more
TLDR
Nucleic acid was prepared by treating wheat streak mosaic virus (WSMV) with sodium dodecyl sulfate and bentonite at pH 9.0 and was susceptible to hydrolysis by 10 −6 μg of ribonuclease per milliliter.About:
This article is published in Virology.The article was published on 1970-11-01. It has received 82 citations till now. The article focuses on the topics: Wheat streak mosaic virus & Tobacco mosaic virus.read more
Citations
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Journal ArticleDOI
Properties and in vitro Translation of Maize Dwarf Mosaic Virus RNA
TL;DR: The RNA of maize dwarf mosaic virus strain A was characterized and compared with that of strain B, and protein profiles from the two RNAs were distinct including translation products that comigrated with authentic coat protein at 37K or 39K for each strain.
Journal ArticleDOI
Characteristics of ribonucleic acids isolated from Botryodiplodia theobromae pycnidiospores
TL;DR: Nucleic acids isolated from dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA, which include two ribosomal species, two “ribosomal-associated” species and transfer RNAs, and polyadenylate-containing RNA.
Journal ArticleDOI
Some characteristics of infectious RNA from potato virus Y.
TL;DR: Infectious potato virus Y-RNA behaved as a single component in sucrose density gradient centrifugation and in polyacrylamide gel electrophoresis and was estimated by extrapolation to be 3.2 million daltons.
Journal ArticleDOI
Biological and physico-chemical properties of an atypical mutant of tobacco mosaic virus.
TL;DR: Rate zonal sucrose density-gradient and equilibrium sedimentations in sucrose and CsCl indicate that the purified mutant preparations contain a heterogenous population of particles including some “free” coat protein, and observations and other data indicate thatThe mutant particles are inherently unstable and break during the purification procedure.
Journal ArticleDOI
Cytological changes induced by wheat streak mosaic virus in cereal leaf tissues
Jian-Guo Gao,Annette Nassuth +1 more
TL;DR: In mesophyll cells of wheat, barley, and triticale, virus infection induced dense cytoplasm around enlarged nuclei and cytopathie structures within these nuclei, which changed and included deposits that were most likely phenolic compounds and lignin.
References
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Journal ArticleDOI
Purification of viral RNA by means of bentonite.
TL;DR: A technique is described for the isolation of tobacco mosaic virus (TMV) ribonucleic acid (RNA) of higher purity and, as will be shown in the subsequent paper, of increased stability.
Book ChapterDOI
Assembly and Stability of the Tobacco Mosaic Virus Particle
TL;DR: The structural and physical chemical studies on TMV considered in this chapter provide a picture of the way in which the viral nucleic acid is packaged for transmission, and illustrate some general principles regarding the molecular organization of biological structures.
Journal ArticleDOI
Spectrophotometric determination of microgram quantities of protein without nucleic acid interference
TL;DR: A spectrophotometric method which eliminates interference due to nucleic acid absorbance has been developed for determining protein concentration over the range of 5 to 180 μg/ml and the results obtained have been compared to those obtained with the Folin-Lowry and microbiuret methods.
Journal ArticleDOI
Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.
TL;DR: Reaction with formaldehyde has been used to denature the bihelical regions of RNA and convert the polynucleotides into random coils characterized by a single equation relating the logarithm of the sedimentation coefficient to that of the molecular weight.
Book ChapterDOI
Some Problems Concerning the Macromolecular Structure of Ribonucleic Acids
TL;DR: The chapter describes that phenol deproteinization involves the treatment of biological material with water-saturated phenol, provides immediate and practically complete inactivation of nucleases, causes no degradation of polyribonucleotide chains during the isolation procedure, completely removes the proteins, and thus secures a high purity of the RNA preparation.
Related Papers (5)
Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids
Myron K. Brakke,Nancy Van Pelt +1 more