Journal ArticleDOI
Purification of viral RNA by means of bentonite.
TLDR
A technique is described for the isolation of tobacco mosaic virus (TMV) ribonucleic acid (RNA) of higher purity and, as will be shown in the subsequent paper, of increased stability.About:
This article is published in Virology.The article was published on 1961-05-01. It has received 608 citations till now. The article focuses on the topics: Tobacco mosaic virus & RNA.read more
Citations
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Isolation and characterization of ribosomal ribonucleic acid
TL;DR: The ribosomal RNA is stable in the presence of concentrated salt solution and, although the secondary structure is lost by heating at 70° in 10mm-sodium acetate, it can be re-formed in the Presence of 200mm-Sodium acetates.
Journal ArticleDOI
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.
TL;DR: base composition and kinetics data strongly suggest that no relationship of precursor-to-product type or, at any rate, not a simple one, exists between the heavy non-ribosomal type nuclear RNA and the cytoplasmic messenger RNA.
Journal ArticleDOI
Rapidly labeled HeLa cell nuclear RNA: I. Identification by zone sedimentation of a heterogeneous fraction separate from ribosomal precursor RNA
TL;DR: The rapidly labeled RNA from HeLa cells has been further studied in this article, showing that 50% of the [3H]uridine incorporated into RNA in four to five minutes is found in this heterogeneous fraction.
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Studies on the stimulation by ammonium sulphate of the DNA-dependent RNA polymerase of isolated rat-liver nuclei.
C.C. Widnell,Jamshed R. Tata +1 more
TL;DR: It is inferred that nuclei incubated in the absence of ammonium sulphate may synthesize ribosomal RNA, and that the action of ammonia sulphate is to induce the synthesis of an RNA more DNA-like in its base composition.
Journal ArticleDOI
A comparison of the killer character in different yeasts and its classification.
T. W. Young,M. Yagiu +1 more
TL;DR: The killer character of the 3 killing groups of Saccharomyces identified could be cured by treatment with cycloheximide or incubation at elevated temperature and the effectiveness of these procedures was indicative of the category of killer yeast examined.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
A paper chromatographic method for the quantitative estimation of amino-acids.
Journal ArticleDOI
Virus reconstitution. II. Combination of protein and nucleic acid from different strains.
Heinz Fraenkel-Conrat,B Singer +1 more
TL;DR: The dual nature of the activity of reconstituted virus particles has thus been clearly demonstrated andVariants or mutants of different biological and chemical properties have occurred randomly in the course of this work, and are regarded as indications of a labilisation of the genetic material through chemical manipulation.
Journal ArticleDOI
The inactivation of ribonuclease during the isolation of ribonucleic acids and ribonucleoproteins from yeast.
TL;DR: The object of the present work was to find inhibitors suitable for this purpose and Copper and Zn2+ ions inhibit pancreatic ribonuclease but, as the present investigations have shown, these have limited use in isolation procedures for ribon nucleic acids and ribonuclebproteins.
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