Journal ArticleDOI
Properties of infectious ribonucleic acid from wheat streak mosaic virus.
Myron K. Brakke,Nancy Van Pelt +1 more
TLDR
Nucleic acid was prepared by treating wheat streak mosaic virus (WSMV) with sodium dodecyl sulfate and bentonite at pH 9.0 and was susceptible to hydrolysis by 10 −6 μg of ribonuclease per milliliter.About:
This article is published in Virology.The article was published on 1970-11-01. It has received 82 citations till now. The article focuses on the topics: Wheat streak mosaic virus & Tobacco mosaic virus.read more
Citations
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Journal ArticleDOI
Some properties of the protein and nucleic acid of oat necrotic mottle virus
TL;DR: In this paper, the protein and nucleic acid properties of oat necrotic mottle virus were investigated and some properties of the virus were discussed, including the properties of proteins and nuclei.
Journal ArticleDOI
Properties of a solubilized replicase isolated from corn infected with maize dwarf mosaic virus
TL;DR: An RNA-dependent RNA polymerase (replicase) activity which catalyzes the polymerization of ribonucleotides into an acid-insoluble product has been isolated and solubilized from the leaves of maize dwarf mosaic virus infected maize.
Journal ArticleDOI
Wheat streak mosaic virus-induced cytoplasmic expansion and membrane proliferation
Jian-Guo Gao,A. Nassuth +1 more
TL;DR: The presence of apparently intermediate stages in myelin-like structure development in chloroplasts suggest that at least some of the myelin of wheat streak mosaic virus-infected cells originated from the chloroplAST.
Journal ArticleDOI
Efficacy of Various Procedures in Isolating Ribonucleic Acid from Plant Virions Exposed to Selected Chemical or Physical Agents1)
Jong-ho Jean,O. P. Sehgal +1 more
Book ChapterDOI
Viruses of the Potyviridae with non-aphid vectors.
TL;DR: Viruses of the Potyviridae induce cytoplasmic cylindrical inclusions in their hosts whether their vectors are aphids, non-aphids, or are unknown, and these viruses have related inclusion body gene sequences.
References
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Journal ArticleDOI
Purification of viral RNA by means of bentonite.
TL;DR: A technique is described for the isolation of tobacco mosaic virus (TMV) ribonucleic acid (RNA) of higher purity and, as will be shown in the subsequent paper, of increased stability.
Book ChapterDOI
Assembly and Stability of the Tobacco Mosaic Virus Particle
TL;DR: The structural and physical chemical studies on TMV considered in this chapter provide a picture of the way in which the viral nucleic acid is packaged for transmission, and illustrate some general principles regarding the molecular organization of biological structures.
Journal ArticleDOI
Spectrophotometric determination of microgram quantities of protein without nucleic acid interference
TL;DR: A spectrophotometric method which eliminates interference due to nucleic acid absorbance has been developed for determining protein concentration over the range of 5 to 180 μg/ml and the results obtained have been compared to those obtained with the Folin-Lowry and microbiuret methods.
Journal ArticleDOI
Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.
TL;DR: Reaction with formaldehyde has been used to denature the bihelical regions of RNA and convert the polynucleotides into random coils characterized by a single equation relating the logarithm of the sedimentation coefficient to that of the molecular weight.
Book ChapterDOI
Some Problems Concerning the Macromolecular Structure of Ribonucleic Acids
TL;DR: The chapter describes that phenol deproteinization involves the treatment of biological material with water-saturated phenol, provides immediate and practically complete inactivation of nucleases, causes no degradation of polyribonucleotide chains during the isolation procedure, completely removes the proteins, and thus secures a high purity of the RNA preparation.
Related Papers (5)
Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids
Myron K. Brakke,Nancy Van Pelt +1 more