Protection against infertility in a BALB/c mouse salpingitis model by intranasal immunization with the mouse pneumonitis biovar of Chlamydia trachomatis.
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Female BALB/c mice were immunized intranasally with the mouse pneumonitis biovar of Chlamydia trachomatis and subsequently challenged in the ovarian bursa and mounted a significant immune response to chlamydial antigens, as shown by a lymphocyte proliferation assay, compared with the sham-immunized nonchallenged mice.Abstract:
Female BALB/c mice were immunized intranasally with the mouse pneumonitis biovar of Chlamydia trachomatis and subsequently challenged in the ovarian bursa (C. trachomatis immunized, C. trachomatis challenged). Two groups of mice served as controls. One group was sham immunized intranasally with mock-infected HeLa 229 cell extracts and was challenged in the ovarian bursa with C. trachomatis MoPn (sham immunized, C. trachomatis challenged). The second control group was sham immunized and not challenged (sham immunized, nonchallenged). Before challenge, the C. trachomatis-immunized, C. trachomatis-challenged animals mounted a significant humoral response as shown by high immunoglobulin G (IgG), IgM, and IgA levels and high levels of neutralizing antibodies in serum and moderate IgG and IgA titers in vaginal secretions. Reactivity by Western blot (immunoblot) to the lipopolysaccharide, 30-, 40- (major outer membrane protein), and 60-kDa cysteine-rich proteins and 75- and 100-kDa chlamydial components could be demonstrated. However, reactivity to the 60-kDa heat shock protein was only observed 22 days after challenge. In addition, this group of animals mounted a significant immune response to chlamydial antigens, as shown by a lymphocyte proliferation assay, compared with the sham-immunized nonchallenged mice. After intrabursal challenge, there was no C. trachomatis shedding from the vagina in the C. trachomatis-immunized, C. trachomatis-challenged animals, while 63% of the sham-immunized, C. trachomatis-challenged mice had a positive C. trachomatis culture. In addition, histological sections from the genital tract showed, at 2 weeks postchallenge, a marked acute inflammatory reaction in the sham-immunized, C. trachomatis-challenged animals while in the C. trachomatis-immunized, C. trachomatis-challenged mice there was minimal inflammatory reaction. When the animals were mated, only 12% of the mice from the sham-immunized, C. trachomatis-challenged mice were fertile. In contrast, 94 and 80% of the sham-immunized, nonchallenged and C. trachomatis-immunized, C. trachomatis-challenged mice, respectively, were fertile.read more
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Imaging of Effector Memory T Cells during a Delayed-Type Hypersensitivity Reaction and Suppression by Kv1.3 Channel Block
Melanie P. Matheu,Christine Beeton,Adriana Garcia,Victor Chi,Srikant Rangaraju,Olga Safrina,Kevin Monaghan,Marc Uemura,Dan Li,Sukumar Pal,Luis M. de la Maza,Edwin S. Monuki,Alexander Flügel,Michael W. Pennington,Ian Parker,K. George Chandy,Michael D. Cahalan +16 more
TL;DR: Results demonstrate a requirement for Kv1.3 channels in Tem cells during an inflammatory immune response in peripheral tissues and allow for effector memory responses to be suppressed while central memory responses remain intact.
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Male Genital Tract Chlamydial Infection: Implications for Pathology and Infertility
TL;DR: Examination of responses to infection in humans and in experimental animal models suggest that an immunoglobulin A-inducing vaccine will be able to target the male reproductive tract effectively while avoiding harmful inflammatory responses that may impair fertility.
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Vaccination with the Chlamydia trachomatis Major Outer Membrane Protein Can Elicit an Immune Response as Protective as That Resulting from Inoculation with Live Bacteria
TL;DR: In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice.
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Histopathologic changes related to fibrotic oviduct occlusion after genital tract infection of mice with Chlamydia muridarum.
Anita A. Shah,Justin H. Schripsema,Mohammad T. Imtiaz,Ira M. Sigar,John N. Kasimos,Peter G. Matos,Sandra Inouye,Kyle H. Ramsey +7 more
TL;DR: Intralumenal occluding fibrosis of the oviduct is a sequela of infection with C. muridarum in this model and support the use of the murine model to study pathogenesis of chlamydial upper genital tract infection.
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Long-Term Immunity and Protection against Herpes Simplex Virus Type 2 in the Murine Female Genital Tract after Mucosal but Not Systemic Immunization
TL;DR: Mucosal immunization provides a high and long-lasting level of immunity from sexually transmitted viral infections of the female genital tract, and examination of antibody-secreting cells during the decline in vaginal virus titers revealed that gB-specific IgA ASCs were only observed in the genital tissues of i.nl.
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