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Protein and peptide factors from Bacillus sp.739 inhibit the growth of phytopathogenic fungi

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TLDR
Thermolabile peptides inhibiting the growth of Helminthosporium sativum, a facultative phytopathogen, have been isolated from the low-molecular-weight fraction of extracellular metabolites of the strain Bacillus sp.
Abstract
Thermolabile peptides inhibiting the growth of Helminthosporium sativum, a facultative phytopathogen, have been isolated from the low-molecular-weight fraction of extracellular metabolites of the strain Bacillus sp. 739. Paper chromatography of the fraction, followed by bioautography, revealed the presence of three components exhibiting antifungal activity. These components were separated by gel chromatography on Toyopearl HW-40. SDS-Na-PAGE (the Laemmli procedure) demonstrated that only one component was a protein (MW, ∼14 kDa). The other two substances were polypeptides with molecular weights less than 6 kDa each. The protein factor inhibited the growth of H. sativum with a minimum effective concentration of 0.1 to 0.2 mg/ml.

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Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase

TL;DR: Bacillus subtilis W-118, a strain that produces antifungal materials, excreted a chitinase when cultured in a medium containing shrimp- and crab-shell powder as the major carbon source, which had the unique characteristics of a low molecular mass and acidic pI.
Journal ArticleDOI

A new bacterial strain, Pseudomonas koreensis IB-4, as a promising agent for plant pathogen biological control

TL;DR: P. koreensis strain IB-4 was shown to possess the valuable features characteristic of PGPR microorganisms: antifungal and nitrogenase activities and ability to synthesize indole-3-acetic acid (IAA) and cytokinin-like compounds.

Extracellular Metabolites Produced by a Novel Strain, Bacillus alvei NRC-14: 5. Multiple Plant-Growth Promoting Properties

TL;DR: The results suggest that the strain Bacillus alvei NRC-14 may has potential to be considered as a potent biocontrol agent, effective against several plant diseases, pest insects, and plant parasitic nematodes due to its antagonistic characteristics and multiple plant-growth promoting properties.
Journal ArticleDOI

Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo

TL;DR: The co-culturing between bacteria and fungi may constitute a mechanism to rapidly obtain resistant fungal forms for host defenses, especially for chromoblastomycosis (CBM) experimental infections.

Antifungal activity of Bacillus sp. E164 against Bipolaris sorokiniana.

TL;DR: Control exerted by Bacillus E164 over B. sorokiniana isolates was relevant in vitro, nevertheless the influence and importance of the metabolites produced must be elucidated for the application in vivo.
References
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Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI

Chitinolytic enzymes produced by trichoderma harzianum; antifungal activity of purified endochitinase and chitobiosidase

TL;DR: Two chitinolytic enzymes from Trichoderma harzianum strain P1 were tested for their antifungal activity in bioassays against nine different fungal species and inhibition of spores germination and germ tube elongation was proportional to the level of chitin in the cell wall of the target fungi.
Journal ArticleDOI

Pseudomonas stutzeri YPL-1 Genetic Transformation and Antifungal Mechanism against Fusarium solani, an Agent of Plant Root Rot

TL;DR: In several biochemical tests with culture filtrates of P. stutzeri YPL-1 and in mutational analyses of antifungal activities of reinforced or defective mutants, it is found that the anti-F.
Journal ArticleDOI

Multi-target and medium-independent fungal antagonism by hydrolytic enzymes in Paenibacillus polymyxa and Bacillus pumilus strains from barley rhizosphere

TL;DR: Despite different strategies of enzyme production in the media, both the P. polymyxa and B. pumilus strains demonstrate a multi-target and medium-independent type of fungal antagonism, which is promising for application in biological control.
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