Journal ArticleDOI
Protein sorption to charged microgels: characterizing binding isotherms and driving forces.
Cemil Yigit,Cemil Yigit,Nicole Welsch,Nicole Welsch,Matthias Ballauff,Matthias Ballauff,Joachim Dzubiella,Joachim Dzubiella +7 more
TLDR
A set of Langmuir binding models in which electrostatic cooperativity effects to protein sorption is incorporated in the spirit of Guoy-Chapman-Stern models, where the global substrate (microgel) charge state is modified by bound reactants (charged proteins).Abstract:
We present a set of Langmuir binding models in which electrostatic cooperativity effects to protein sorption is incorporated in the spirit of Guoy-Chapman-Stern models, where the global substrate (microgel) charge state is modified by bound reactants (charged proteins). Application of this approach to lysozyme sorption to oppositely charged core-shell microgels allows us to extract the intrinsic, binding affinity of the protein to the gel, which is salt concentration independent and mostly hydrophobic in nature. The total binding affinity is found to be mainly electrostatic in nature, changes many orders of magnitude during the sorption process, and is significantly influenced by osmotic deswelling effects. The intrinsic binding affinity is determined to be about 7 k(B)T for our system. We additionally show that Langmuir binding models and those based on excluded-volume interactions are formally equivalent for low to moderate protein packing, if the nature of the bound state is consistently defined. Having appreciated this, a more quantitative interpretation of binding isotherms in terms of separate physical interactions is possible in the future for a wide variety of experimental approaches.read more
Citations
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Journal ArticleDOI
Protein interactions with polymer coatings and biomaterials.
Qiang Wei,Tobias Becherer,Stefano Angioletti-Uberti,Joachim Dzubiella,Christian Wischke,Axel T. Neffe,Andreas Lendlein,Matthias Ballauff,Rainer Haag +8 more
TL;DR: Current analytical methods to test mechanistic hypotheses and theories of protein-surface interactions will be discussed and special focus will be given to state-of-the-art bioinert and biospecific coatings and their applications in biomedicine.
Journal ArticleDOI
Dual-stimuli-sensitive microgels as a tool for stimulated spongelike adsorption of biomaterials for biosensor applications.
Larisa V. Sigolaeva,Snezhana Yu. Gladyr,Arjan P. H. Gelissen,Olga Mergel,Dmitry V. Pergushov,Ilya N. Kurochkin,Felix A. Plamper,Walter Richtering +7 more
TL;DR: A very simple, novel, and fast strategy for physical entrapment of biomolecules by the polymeric matrix was proposed and tested and stimulated spongelike adsorption provides polymer films comprising concentrated biomaterial.
Journal ArticleDOI
Isothermal titration calorimetry as a complementary method for investigating nanoparticle–protein interactions
TL;DR: This minireview concludes that nanomaterials formed of a hydrophilic material without strongly charged surface and steric stabilization experience the weakest interactions with proteins and are most promising for allowing an engineering of the protein corona.
Journal ArticleDOI
Adsorption of proteins to functional polymeric nanoparticles
Nicole Welsch,Yan Lu,Joachim Dzubiella,Joachim Dzubiella,Matthias Ballauff,Matthias Ballauff +5 more
TL;DR: In this paper, the authors review the application of various techniques to monitor adsorption of proteins to colloidal particles, including calorimetry, fluorescence spectroscopy and small-angle X-ray scattering.
Journal ArticleDOI
Theory, simulations and the design of functionalized nanoparticles for biomedical applications: A Soft Matter Perspective
TL;DR: A critical overview of theoretical and computational coarse-grained models, developed to describe functionalised nanoparticles’ interactions with the biological environment, are provided and some of the latest results in this fascinating area of research are presented.
References
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Rapid measurement of binding constants and heats of binding using a new titration calorimeter.
TL;DR: A new titration calorimeter is described and results are presented for the binding of cytidine 2'-monophosphate (2'CMP) to the active site of ribonuclease A.
Journal ArticleDOI
Nanoengineering of particle surfaces.
TL;DR: In this article, the authors present the state-of-the-art in strategies for engineering particle surfaces, such as the layer-by-layer deposition process, which allows fine control over shell thickness and composition.
Journal ArticleDOI
Stimuli responsive polymers for biomedical applications
TL;DR: This critical review of polymers that can respond to external stimuli considers the types of stimulus response used in therapeutic applications and the main classes of responsive materials developed to date.