Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.
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Reduction and alkylation of isolated proteoglycan subunit and of the glycoprotein fraction suggest that disulfide bonds in both are required for aggregation, but that those in the former are more sensitive to reduction.About:
This article is published in Journal of Biological Chemistry.The article was published on 1969-05-10 and is currently open access. It has received 570 citations till now. The article focuses on the topics: Guanidinium chloride & Hexuronate.read more
Citations
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Journal ArticleDOI
Material properties and structure-function relationships in the menisci.
TL;DR: The menisci serve several important biomechanical functions in the knee: distribute stresses over a broad area of articular cartilage, absorb shocks during dynamic loading, and probably assist in joint lubrication.
Journal ArticleDOI
The specific interaction of hyaluronic acid with cartilage proteoglycans
Timothy E. Hardingham,Helen Muir +1 more
TL;DR: The addition of small amounts of hyaluronic acid to disaggregated cartilage proteoglyans produced a large increase in hydrodynamic size on gel chromatography and there was no such interaction when proteoglycans were mixed with other polyanions in comparable proportions.
Journal ArticleDOI
Regular ArticleCartilage proteoglycans
Cheryl B. Knudson,Warren Knudson +1 more
TL;DR: The predominant proteoglycans present in cartilage is the large chondroitin sulfate proteoglycan 'aggrecan', which serves a direct, primary role providing the osmotic resistance necessary for cartilage to resist compressive loads.
Journal ArticleDOI
Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
TL;DR: Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.
Journal ArticleDOI
Aggregation of Cartilage Proteoglycans I. THE ROLE OF HYALURONIC ACID
TL;DR: Evidence is presented which suggests that the hyaluronic acid-proteoglycan interactions described by Hardingham and Muir ((1972) Biochim.
References
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The estimation of carbohydrates in plant extracts by anthrone.
E. W. Yemm,A. J. Willis +1 more
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Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
Journal ArticleDOI
Proteinpolysaccharide Complex from Bovine Nasal Cartilage A COMPARISON OF LOW AND HIGH SHEAR EXTRACTION PROCEDURES
TL;DR: Two procedures for isolating 80 to 85% of the total hexuronic acid from bovine nasal cartilage as proteinpolysaccharide complex are described and compared, and it is suggested that disaggregation is fundamental to the extraction process.
Journal ArticleDOI
Viscosity and density of aqueous solutions of urea and guanidine hydrochloride.
Kazuo Kawahara,Charles Tanford +1 more
TL;DR: Densities and viscosities of aqueous solutions of urea and guanidine hydrochloride at 25° have been measured and equations are presented which describe their variation with concentration, and the possibility of predicting these results from information on solutions containing each solute separately is discussed.
Book ChapterDOI
The Viscosity Of Macromolecules In Relation To Molecular Conformation
TL;DR: This chapter discusses the theoretical and experimental aspects of both Newtonian and non-Newtonian viscosities of polymer solutions and explores that viscometry has been one of the most powerful tools for detecting the conformational changes or “denaturation” of proteins, but intimate details of the process cannot be deduced from the viscosity alone.
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Proteinpolysaccharide Complex from Bovine Nasal Cartilage A COMPARISON OF LOW AND HIGH SHEAR EXTRACTION PROCEDURES
The specific interaction of hyaluronic acid with cartilage proteoglycans
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