Proteinpolysaccharide Complex from Bovine Nasal Cartilage A COMPARISON OF LOW AND HIGH SHEAR EXTRACTION PROCEDURES
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TLDR
Two procedures for isolating 80 to 85% of the total hexuronic acid from bovine nasal cartilage as proteinpolysaccharide complex are described and compared, and it is suggested that disaggregation is fundamental to the extraction process.About:
This article is published in Journal of Biological Chemistry.The article was published on 1969-01-10 and is currently open access. It has received 598 citations till now.read more
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Book ChapterDOI
Preparation and characterization of the different types of collagen.
E J Miller,R K Rhodes +1 more
TL;DR: This chapter presents methods generally applicable to the preparation of native collagen from a variety of sources, conducted at relatively low temperatures, in the range of 4-8 °C, to minimizes bacterial growth, enhances the solubility of native collagens, and ensures the retention of native conformation on the part of the Solubilized Collagens.
Journal ArticleDOI
Structure and function of aggrecan
TL;DR: ABSTRACTAggrecan is the major proteoglycan in the articular cartilage because it provides a hydrated gel structure (via its interaction with hyaluronan and link protein) that endows the cartilage with load-bearing properties and is crucial in chondroskeletal morphogenesis during development.
Journal ArticleDOI
The specific interaction of hyaluronic acid with cartilage proteoglycans
Timothy E. Hardingham,Helen Muir +1 more
TL;DR: The addition of small amounts of hyaluronic acid to disaggregated cartilage proteoglyans produced a large increase in hydrodynamic size on gel chromatography and there was no such interaction when proteoglycans were mixed with other polyanions in comparable proportions.
Journal ArticleDOI
Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.
TL;DR: Reduction and alkylation of isolated proteoglycan subunit and of the glycoprotein fraction suggest that disulfide bonds in both are required for aggregation, but that those in the former are more sensitive to reduction.
References
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A modified uronic acid carbazole reaction
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TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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The estimation of carbohydrates in plant extracts by anthrone.
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Journal ArticleDOI