Pseudouridines in spliceosomal snRNAs.
Andrew T. Yu,Junhui Ge,Yi-Tao Yu +2 more
TLDR
Experimental data indicate that virtually all pseudouridines in U2 snRNA are functionally important, strongly suggesting that pseudoursouridylation is also a regulatory modification.Abstract:
Spliceosomal RNAs are a family of small nuclear RNAs (snRNAs) that are essential for pre-mRNA splicing. All vertebrate spliceosomal snRNAs are extensively pseudouridylated after transcription. Pseudouridines in spliceosomal snRNAs are generally clustered in regions that are functionally important during splicing. Many of these modified nucleotides are conserved across species lines. Recent studies have demonstrated that spliceosomal snRNA pseudouridylation is catalyzed by two different mechanisms: an RNA-dependent mechanism and an RNA-independent mechanism. The functions of the pseudouridines in spliceosomal snRNAs (U2 snRNA in particular) have also been extensively studied. Experimental data indicate that virtually all pseudouridines in U2 snRNA are functionally important. Besides the currently known pseudouridines (constitutive modifications), recent work has also indicated that pseudouridylation can be induced at novel positions under stress conditions, thus strongly suggesting that pseudouridylation is also a regulatory modification.read more
Citations
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Journal ArticleDOI
Transcriptome-wide Mapping Reveals Widespread Dynamic-Regulated Pseudouridylation of ncRNA and mRNA
Schraga Schwartz,Douglas A. Bernstein,Maxwell R. Mumbach,Marko Jovanovic,Rebecca H. Herbst,Brian X. León-Ricardo,Jesse M. Engreitz,Mitchell Guttman,Rahul Satija,Eric S. Lander,Gerald R. Fink,Aviv Regev,Aviv Regev,Aviv Regev +13 more
TL;DR: In this paper, the authors develop Ψ-seq for transcriptome-wide quantitative mapping of pseudouridine and identify hundreds of unique sites in human and yeast mRNAs and snoRNAs.
Transcriptome-wide Mapping Reveals Widespread Dynamic-Regulated Pseudouridylation of ncRNA and mRNA
Schraga Schwartz,Douglas A. Bernstein,Maxwell R. Mumbach,Marko Jovanovic,Rebecca H. Herbst,Brian X. León-Ricardo,Jesse M. Engreitz,Mitchell Guttman,Rahul Satija,Eric S. Lander,Gerald R. Fink,Aviv Regev,Aviv Regev,Aviv Regev +13 more
TL;DR: This work identifies an enhanced, transcriptome-wide scope for pseudouridine and methods to dissect its underlying mechanisms and function and discovers hundreds of unique sites in human and yeast mRNAs and snoRNAs.
Journal ArticleDOI
Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome.
TL;DR: This work developed N3-CMC-enriched pseudouridine sequencing (CeU-Seq), a selective chemical labeling and pulldown method, to identify 2,084 Ψ sites within 1,929 human transcripts, of which four (in ribosomal RNA and EEF1A1 mRNA) are biochemically verified.
Journal ArticleDOI
Transcriptome-wide mapping of pseudouridines: pseudouridine synthases modify specific mRNAs in S. cerevisiae.
TL;DR: It is established that site-specific pseudouridylation of eukaryotic mRNAs is a genetically programmed RNA modification that naturally occurs in multiple yeast transcripts via distinct mechanisms, suggesting that mRNA pseudourIDylation may provide an important novel regulatory function.
Journal ArticleDOI
RNA pseudouridylation: new insights into an old modification
Junhui Ge,Yi-Tao Yu +1 more
TL;DR: It has been reported that pseudouridine can be artificially introduced into mRNA by box H/ACA RNPs and that such introduction can mediate nonsense-to-sense codon conversion, thus demonstrating a new means of generating coding or protein diversity.
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