Journal ArticleDOI
Purification and binding characteristics of a basic fatty acid binding protein from Avena sativa seedlings
TLDR
A fatty acid binding protein has been purified from etiolated Avena sativa seedlings and is capable of binding long-chain fatty acids and their CoA-esters.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1984-07-06. It has received 36 citations till now. The article focuses on the topics: Isoelectric focusing & Isoelectric point.read more
Citations
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Journal ArticleDOI
Cell-specific expression of the carrot EP2 lipid transfer protein gene.
TL;DR: A role for plant lipid transfer proteins in the transport of cutin monomers through the extracellular matrix to sites of Cutin synthesis is proposed.
Book ChapterDOI
The cellular fatty acid binding proteins: aspects of structure, regulation, and function.
TL;DR: This chapter provides a comprehensive overview of the current state of knowledge of the research with particular emphasis on evolving concepts of fatty acid binding proteins (FABP) structure, regulation, and function.
Book ChapterDOI
8 – Triacylglycerol Biosynthesis
Sten Stymne,A K Stobart +1 more
TL;DR: In this paper, the microsomal membrane from the developing oil seed offers a fundamental system that, because of its specialized nature, is most abundant in those enzymes associated with lipid metabolism and catalyzes the synthesis of triacylglycerol from glycerol phosphate and regulates the acyl quality of the final oil.
Journal ArticleDOI
High-resolution crystal structure of the non-specific lipid-transfer protein from maize seedlings.
TL;DR: The fold of maize ns-LTP places it in a new category of all-alpha-type structure, first described for soybean hydrophobic protein, which is large enough to accommodate a long fatty acyl chain.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.
R.T. Swank,K.D. Munkres +1 more
TL;DR: Because of the importance of intrinsic charge and conformation, the system, although allowing a first approximation in molecular weight determination, may also be applicable to peptide “mapping,” particularly for “insoluble” peptide mixtures with prominent hydrophobic association, such as encountered in cellular membranes, viruses, and proteolytic digests.
Journal ArticleDOI
A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues.
TL;DR: A protein of molecular weight ∼ 12,000 which binds long-chain fatty acids and certain other lipids has been identified in cytosol of intestinal mucosa, liver, myocardium, adipose tissue, and kidney and appears to be identical with the smaller of two previously described cytoplasmic anion-binding proteins.
Journal ArticleDOI
Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions
TL;DR: Two hepatic cytoplasmic protein fractions, designated Y and Z, which bind sulfobromophthalein (BSP), bilirubin, and other organic anions, have been separated by G75 Sephadex gel filtration and appear to be important in the transfer of Organic anions from plasma into the liver.
Journal ArticleDOI
Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantification.
R K Ockner,J A Manning,J P Kane +2 more
TL;DR: The abundance of FABP, its importance in the cytosolic binding of endogenous as well as exogenous fatty acids, and its demonstrated correlation with rates of hepatocyte fatty acid utilization provide additional evidence for its relationship to the cellular metabolism of long chain fatty acids.