Journal ArticleDOI
Purification and immunochemical studies of pyruvate dehydrogenase complex from rat heart, and cell-free synthesis of lipoamide dehydrogenase, a component of the complex.
TLDR
In cell-free translation programmed with free and membrane-bound polysomes, activity of mRNA coding for the precursor of the enzyme was much higher in freepolysomes than in membrane- bound polysome, and its translation product was detected as a putative precursor larger than the mature subunit.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1983-10-13. It has received 30 citations till now. The article focuses on the topics: Oxoglutarate dehydrogenase complex & Branched-chain alpha-keto acid dehydrogenase complex.read more
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Regulation of mitochondrial pyruvate dehydrogenase activity by tau protein kinase I/glycogen synthase kinase 3beta in brain
Minako Hoshi,Akihiko Takashima,Kaori Noguchi,Miyuki Murayama,Michio Sato,Shunzo Kondo,Yoshito Saitoh,Koichi Ishiguro,Toshimitsu Hoshino,Kazutomo Imahori +9 more
TL;DR: Results suggest that TPKI/GSK-3beta plays a key role in the pathogenesis of Alzheimer disease and regulates PDH and participates in energy metabolism and acetylcholine synthesis.
Journal Article
Autoantibodies of primary biliary cirrhosis recognize dihydrolipoamide acetyltransferase and inhibit enzyme function.
J Van de Water,David R. Fregeau,Paul A. Davis,Aftab A. Ansari,Dean J. Danner,Patrick S.C. Leung,Ross L. Coppel,M. E. Gershwin +7 more
TL;DR: Identification of dihydrolipoamide acetyltransferase as the target of autoimmunity in PBC provides a reagent that can be used to determine mechanisms by which this molecule is recognized, and will allow study of whether autoimmune reactivity, at the humoral or T cell level, is the basis for the pathogenesis of PBC.
Journal ArticleDOI
Dihydrolipoamide dehydrogenase: Functional similarities and divergent evolution of the pyridine nucleotide-disulfide oxidoreductases
TL;DR: The comparison of the complete amino acid sequences of E3 from Escherichia coli, yeast, pig, and human shows considerable homologies of certain amino acid residues or short stretches of sequences, especially in the specific catalytic and structural domains.
Journal ArticleDOI
Isolation and sequence determination of cDNA clones for porcine and human lipoamide dehydrogenase: homology to other disulfide oxidoreductases
G Otulakowski,Brian H. Robinson +1 more
TL;DR: Alignment of the deduced amino acid sequence of human lipoamide dehydrogenase with human erythrocyte glutathione reductase and mercuric reduct enzyme from Tn501 revealed extensive homologies throughout the primary sequence, suggesting that secondary and tertiary structure is also similar among these three enzymes.
Journal ArticleDOI
Cloning and cDNA sequence of the dihydrolipoamide dehydrogenase component human alpha-ketoacid dehydrogenase complexes.
Gabriel Pons,Cindy Raefsky-Estrin,Donna J. Carothers,Ronald A. Pepin,Ali A. Javed,Barry W. Jesse,M K Ganapathi,David Samols,Mulchand S. Patel +8 more
TL;DR: By blot-hybridization analysis with the cDNA as probe, two mRNAs, 2.2 and 2.4 kilobases in size, have been detected in human tissues and fibroblasts, whereas only one mRNA was detected in rat tissues.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.