Journal ArticleDOI
Purification and properties of aldose 1-epimerase from Aspergillus niger.
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TLDR
The time required for glucose determination with a glucose oxidase reagent was significantly shortened by the addition of aldose 1-epimerase.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1981-12-15. It has received 9 citations till now. The article focuses on the topics: Aspergillus niger & Aldose.read more
Citations
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Journal ArticleDOI
The Oligomeric Stromal Proteome of Arabidopsis thaliana Chloroplasts
Jean-Benoît Peltier,Y. Cai,Qi Sun,Vladimir Zabrouskov,L. Giacomelli,Andrea Rudella,A. Jimmy Ytterberg,Heidi Rutschow,Klaas J. van Wijk +8 more
TL;DR: This study presents an analysis of the stromal proteome in its oligomeric state extracted from highly purified chloroplasts of Arabidopsis thaliana and confirmed the accuracy of the native mass determination, and by extension, the usefulness of thenative mass data for future in-depth protein interaction studies.
, model integration of the bibliome, genome, metabolome and reactome of Aspergillus niger
TL;DR: This work presents the largest model reconstruction of a metabolic network reported for a fungal species, based on the reportings of 371 articles and comprises 1190 biochemically unique reactions and 871 ORFs, and a graphical map of the metabolic network is presented.
Journal ArticleDOI
Mechanistic aspects of enzymatic carbohydrate epimerization.
Jomy Samuel,Martin E. Tanner +1 more
TL;DR: This review focuses on the mechanistic strategies that are employed by this diverse family of enzymes, and is divided into the following sections: deprotonation/reprotonation, transient oxidation, cofactor independent, and mutarotation.
Journal ArticleDOI
Flow injection analysis of lactose using covalently immobilized β-galactosidase, mutarotase, and glucose oxidase/peroxidase on a 2-fluoro-1-methylpyridinium salt-activated fractogel support
TL;DR: The FIA method consistently gives the lowest standard deviations and coefficient of variation for the various milk matrices analyzed, and hence could obviate the need for the more expensive mutarotase.
Dissertation
Microbial Production of Glucose Oxidase and its Commercial Applications
TL;DR: The aim of the project was to improve GOX production using mutagenesis of A. niger, to optimize the conditions of fermentation, screen fungal strains producing highest GOX activity, and to optimize commercial applications of glucose oxidase.
References
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Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
The measurement of amino groups in proteins and peptides
TL;DR: A technique is examined for determining amino groups with 2,4,6-trinitrobenzenesulphonic acid, in which the extinction at 420nm of sulphite complexes of the trinitrophenylated amino groups is measured, and the reaction with several amino acids, peptides and proteins is presented.
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