Journal ArticleDOI
Purification, characterization, kinetic properties, and thermal behavior of extracellular polygalacturonase produced by filamentous fungus Tetracoccosporium sp.
TLDR
This enzyme exhibited a t1/2 of 63 min at 60°C and its specific activity, turnover number, and catalytic efficiency were 6.17 U/mg, 113.64 min−1, and 35.18 mL/(min·mg), respectively, revealing a potential application for the industry.Abstract:
For the first time, a polygalacturonase from the culture broth of Tetracoccosporium sp. was isolated and incubated at 30 degrees C in an orbital shaker at 160 rpm for 48 h. The enzyme was purified by ammonium sulfate precipitation and two-step ion-exchange chromatography and had an apparent molecular mass of 36 kDa, as shown by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Its optimum activity was at pH 4.3 and 40 degrees C, and the Km and Vmax values of this enzyme (for polygalacturonic acid) were 3.23 mg/mL and 0.15 micromol/min, respectively. Ag+, Co2+, EDTA, Tween-20, Tween-80, and Triton X-100 stimulated polygalacturonase activity whereas Al3+, Ba2+, Ca2+, Fe2+, Fe3+, Ni2+, Mg2+, Mn2+, and SDS inhibited it. In addition, iodoacetamide and iodoacetic acid did not inhibit enzyme activity at a concentration of 1 mM, indicating that cysteine residues are not part of the catalytic site of polygalacturonase. We studied the kinetic properties and thermal inactivation of polygalacturonase. This enzyme exhibited a t1/2 of 63 min at 60 degrees C and its specific activity, turnover number, and catalytic efficiency were 6.17 U/mg, 113.64 min-1, and 35.18 mL/(min.mg), respectively. The activation energy (DeltaE#) for heat inactivation was 5.341 kJ/mol, and the thermodynamic activation parameters DeltaG#, DeltaH#, and DeltaS# were also calculated, revealing a potential application for the industry.read more
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Journal ArticleDOI
Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A
Mandana Zarei,Saeed Aminzadeh,Hossein Zolgharnein,Alireza Safahieh,Morteza Daliri,Kambiz Akbari Noghabi,Ahmad Ghoroghi,Abbasali Motallebi +7 more
TL;DR: This novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A, revealing a potential application for the industry with potentially exploitable significance and implying that difference in chitinolytic ability must result from the subsite structure in the enzyme binding cleft.
Journal ArticleDOI
Characterization of a Chitinase (Chit62) from Serratia marcescens B4A and Its Efficacy as a Bioshield Against Plant Fungal Pathogens
TL;DR: The recombinant chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters and was demonstrated to be highly active in controlling fungal pathogens.
Journal ArticleDOI
Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
TL;DR: The results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production, indicating a potential application of this enzyme in several industries.
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Serratia marcescens b4a chitinase product optimization using taguchi approach
TL;DR: In this article, a chitinase-producing bacterium was identified as Serratia marcescens B4A based on its 16S rRNA sequence and key morphological, physiological and biochemical characteristics.
Journal ArticleDOI
Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance
Zuming Li,Zuming Li,Zhihui Bai,Baoguo Zhang,Baojv Li,Bo Jin,Michael Zhang,Francis Lin,Hongxun Zhang +8 more
TL;DR: The alkaline pectin lyase (PNL) was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture.
References
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Journal Article
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Teodoro Espinosa-Solares,John Bombardiere,Mark Chatfield,Max Domaschko,Michael Easter,David A. Stafford,Saul Castillo-Angeles,Nehemias Castellanos-Hernandez +7 more
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