Journal ArticleDOI
Purification of docosahexaenoic acid by selective esterification of fatty acids from tuna oil with Rhizopus delemar lipase
Yuji Shimada,Akio Sugihara,Hirofumi Nakano,Takashi Kuramoto,Toshihiro Nagao,Munekazu Gemba,Yoshio Tominaga +6 more
TLDR
To purify docosahexaenoic acid (DHA), the selective esterification of fatty acids originating from tuna oil with lipases was attempted, and DHA was purified to 89% with a recovery of 71% of its initial content.Abstract:
To purify docosahexaenoic acid (DHA), we attempted the selective esterification of fatty acids originating from tuna oil with lipases. Tuna oil was hydrolyzed in NaOH-ethanol solution, and the resulting fatty acid mixture [DHA, 23.2%; named tuna-free fatty acid (FFA)] was used as a starting material. Rhizopus delemar which acted lightly on DHA, was a suitable catalyst for the selective esterification of tuna-FFA, and lauryl alcohol was the best substrate. The reaction proceeded most effectively when a mixture of 2.4 g lauryl alcohol/tuna-FFA (2:1, mol/mol), 0.6 g water, and 600 U Rhizopus lipase was incubated at 30°C for 20 h with stirring at 500 rpm. Under these conditions 72% of tuna-FFA was esterified, and 84% of DHA was recovered in the unesterified fatty acid fraction. The DHA content in the fatty acid fraction rose from 23 to 73% with this reaction. To further elevate the DHA content, the unesterified fatty acids were extracted, and then esterified again under the same conditions. By this repeated esterification, DHA was purified to 89% with a recovery of 71% of its initial content.read more
Citations
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Journal ArticleDOI
Biodiesel fuel production by transesterification of oils.
TL;DR: Biodiesel (fatty acid methyl esters), which is derived from triglycerides by transesterification with methanol, has attracted considerable attention during the past decade as a renewable, biodegradable, and nontoxic fuel.
Journal ArticleDOI
Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase
Yuji Shimada,Yomi Watanabe,Taichi Samukawa,Akio Sugihara,Hideo Noda,Hideki Fukuda,Yoshio Tominaga +6 more
TL;DR: In this paper, the authors attempted continuous methanolysis of vegetable oil by an enzymatic process, which was conducted by adding methanol stepwise to avoid lipase inactivation.
Journal ArticleDOI
Production of specific‐structured triacylglycerols by lipase‐catalyzed reactions: a review
TL;DR: Encouraging products resulting from this regiospecificity are reviewed together with the critical evaluation of their reaction schemes, side reactions and by-products, sources of substrate oils and acyl donors, and production processes.
Journal ArticleDOI
Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit.
TL;DR: The enzyme poorly hydrolyzed triacylglycerols containing n-3 polyunsaturated fatty acids, and appeared as a suitable biocatalyst for selective esterification of sardine free fatty acids with hexanol as substrate, showed a good stability in organic solvents and especially in long chain-fatty alcohol.
Journal ArticleDOI
Lipase-Catalyzed Esterification
Neena N. Gandhi,Nitin Sopanrao Patil,Sudhirprakash B. Sawant,Jyeshtharaj B. Joshi,Pramod P. Wangikar,D. Mukesh +5 more
TL;DR: This review deals with fundamental as well as practical aspects of lipase catalysis, and various immobilization techniques have been studied for lipases and some have been shown to enhance the activity and stability of the enzyme.
References
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Journal ArticleDOI
Fish oils and plasma lipid and lipoprotein metabolism in humans: a critical review.
TL;DR: Fish oil or linolenic acid may serve as alternative sources of long-chain n-3 FAs, but further studies will be needed to document their hypolipidemic and/or antiatherogenic effects.
Journal ArticleDOI
Concentration of docosahexaenoic acid in glyceride by hydrolysis of fish oil withcandida cylindracea lipase
TL;DR: In this paper, the acyl chain specificity of lipases is evaluated in terms of hydrolysis resistant value (HRV), which is the ratio between the DHA contents in the glyceride mixture of the hydrolyzed oil and original oil.
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Lipolysis of menhaden oil triacylglycerols and the corresponding fatty acid alkyl esters by pancreatic lipase in vitro: a reexamination.
TL;DR: It is concluded that in the presence of a large excess of lipase the liberated fatty acids would approach the composition of the dietary alkyl or glyceryl esters, as observed during lumenal lipolysis.
Journal ArticleDOI
Selective Hydrolysis of Fish Oil by Lipase to Concentrate n-3 Polyunsaturated Fatty Acids
TL;DR: Candida cylindracea lipase seems the most promising with respect to recovery of triacylglycerol and Aspergillus niger lipase increased not only the docosahexaenoic acid (DHA) content but also eicosapentaenoic acids (EPA) and docosapentenoic Acid (DPA), although the absolute value of the latter was quite low.
Journal ArticleDOI
Enrichment of polyunsaturated fatty acids withGeotrichum candidum lipase
TL;DR: Geotrichum candidum lipase was found to be suitable for their concentration in glycerides as discussed by the authors, and was used to extract polyunsaturated fatty acids in good yield.