Journal ArticleDOI
Purification of human blood eosinophils by negative selection using immunomagnetic beads.
T.T. Hansel,J.D. Pound,Darrell Pilling,George D. Kitas,Mike Salmon,T.A. Gentle,Shui Shan Lee,R.A. Thompson +7 more
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TLDR
A negative immunoselection produced eosinophils of greater purity and with a superior capacity to mount a respiratory burst than eos inophils isolated by a method employing metrizamide.About:
This article is published in Journal of Immunological Methods.The article was published on 1989-08-15. It has received 201 citations till now. The article focuses on the topics: Dynabeads & Percoll.read more
Citations
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Journal ArticleDOI
Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils.
Paul D. Ponath,Shixin Qin,Douglas J. Ringler,Ian Clark-Lewis,Juan Wang,Nasim Kassam,Heidi Smith,Xiaojie Shi,Jose Angel Gonzalo,Walter Newman,Jose-Carlos Gutierrez-Ramos,Charles R. Mackay +11 more
TL;DR: Immunohistochemistry on human nasal polyp with antieotaxin mAbs showed that certain leukocytes as well as respiratory epithelium were intensely immunoreactive, and eosinophil infiltration occurred at sites of eotAXin upregulation.
Journal ArticleDOI
Adhesion of human basophils, eosinophils, and neutrophils to interleukin 1-activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules.
Bruce S. Bochner,Francis W. Luscinskas,Michael A. Gimbrone,Walter Newman,Sherry A. Sterbinsky,Claudia P. Derse-Anthony,David Klunk,Robert P. Schleimer +7 more
TL;DR: Different roles for ICAM-1, ELAM-2, and VCAM-3 are document during basophil, eosinophils, and neutrophil adhesion in vitro, and a novel mechanism for the recruitment of eos inophils and basophils to sites of inflammation in vivo is suggested.
Journal ArticleDOI
Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein
Donald B. Carter,Martin R. Deibel,Colin J. Dunn,Che-Shen C. Tomich,Alice L. Laborde,Jerry L. Slightom,Ann E. Berger,Michael J. Bienkowski,Frank F. Sun,Robert N. McEwan,Peter K. W. Harris,Anthony W. Yem,G. A. Waszak,J G Chosay,L. C. Sieu,M. M. Hardee,H. A. Zurcher-Neely,I M Reardon,Robert L. Heinrikson,Scott E. Truesdell,J. A. Shelly,Thomas E. Eessalu,B. M. Taylor,Daniel E. Tracey +23 more
TL;DR: A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced and was a potent inhibitor of IL-1 induced corticosterone production in vivo.
Journal ArticleDOI
Endogenous airway acidification. Implications for asthma pathophysiology.
John F. Hunt,Kezhong Fang,Rajesh K. Malik,Ashley H. Snyder,Neil R. Malhotra,Thomas A.E. Platts-Mills,Benjamin Gaston +6 more
TL;DR: It is demonstrated that the pH of deaerated exhaled airway vapor condensate is over two log orders lower in patients with acute asthma than in control subjects and normalizes with corticosteroid therapy, and suggested that regulation of airway pH has a previously unsuspected role in asthma pathophysiology.
Journal ArticleDOI
An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils
TL;DR: The immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophil isolation after N-formyl-methionyl-leucyl-phenyl-alanine treatment.
References
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Journal ArticleDOI
The role of protein kinase C in cell surface signal transduction and tumour promotion
TL;DR: Protein kinase C probably serves as a receptor for the tumour promoters and further exploration of the roles of this enzyme may provide clues for understanding the mechanism of cell growth and differentiation.
Journal Article
A New Method for the Purification of Human Eosinophils and Neutrophils, and a Comparison of the Ability of These Cells to Damage Schistosomula of Schistosoma Mansoni
TL;DR: Centrifugation of human white blood cells over either Ficoll-Hypaque or slightly hypertonic Metrizamide discontinuous gradients reliably produces separate fractions that are enriched for either neutrophilic or eosinophilic granulocytes.
Journal ArticleDOI
Increased numbers of hypodense eosinophils in the blood of patients with bronchial asthma.
T. Fukuda,S. L. Dunnette,Charles E. Reed,Steven J. Ackerman,Margot S. Peters,Gerald J. Gleich +5 more
TL;DR: The density of blood eosinophils from patients with asthma using polyvinylpyrrolidone-coated silica gel (Percoll) discontinuous density gradient centrifugation of peripheral blood leukocytes was investigated and it was found that a portion of the eosInophils in asthmatic patients and most of the Eos in patients with HES are hypodense.
Journal Article
Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll).
TL;DR: The separation technique did not affect the viability or the metabolic capacities of the cells and the purity ranged from 86 to 99% eosinophils in the final preparation.
Book ChapterDOI
[8] Purification and cryopreservation of phagocytes from human blood
Dirk Roos,Martin de Boer +1 more
TL;DR: Three types of phagocytic cell are found in human blood: monocytes, neutrophilic granulocytes, and eosinophilicgranulocytes; both biological and physical properties of the cells can be used for their separation.