PYK10 myrosinase reveals a functional coordination between endoplasmic reticulum bodies and glucosinolates in Arabidopsis thaliana.
Ryohei Thomas Nakano,Ryohei Thomas Nakano,Mariola Piślewska-Bednarek,Kenji Yamada,Patrick P. Edger,Mado Miyahara,Maki Kondo,Christoph Böttcher,Masashi Mori,Mikio Nishimura,Paul Schulze-Lefert,Ikuko Hara-Nishimura,Paweł Bednarek +12 more
TLDR
The authors showed that PYK10, the most abundant β-glucosidase in A.thaliana root ER bodies, hydrolyzes indole glucosinolates (IGs) in addition to the previously reported in vitro substrate scopolin.Abstract:
The endoplasmic reticulum body (ER body) is an organelle derived from the ER that occurs in only three families of the order Brassicales and is suggested to be involved in plant defense. ER bodies in Arabidopsis thaliana contain large amounts of β-glucosidases, but the physiological functions of ER bodies and these enzymes remain largely unclear. Here we show that PYK10, the most abundant β-glucosidase in A. thaliana root ER bodies, hydrolyzes indole glucosinolates (IGs) in addition to the previously reported in vitro substrate scopolin. We found a striking co-expression between ER body-related genes (including PYK10), glucosinolate biosynthetic genes and the genes for so-called specifier proteins affecting the terminal products of myrosinase-mediated glucosinolate metabolism, indicating that these systems have been integrated into a common transcriptional network. Consistent with this, comparative metabolite profiling utilizing a number of A. thaliana relatives within Brassicaceae identified a clear phylogenetic co-occurrence between ER bodies and IGs, but not between ER bodies and scopolin. Collectively, our findings suggest a functional link between ER bodies and glucosinolate metabolism in planta. In addition, in silico three-dimensional modeling, combined with phylogenomic analysis, suggests that PYK10 represents a clade of 16 myrosinases that arose independently from the other well-documented class of six thioglucoside glucohydrolases. These findings provide deeper insights into how glucosinolates are metabolized in cruciferous plants and reveal variation of the myrosinase-glucosinolate system within individual plants.read more
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Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants
Ivica Blažević,Sabine Montaut,Franko Burčul,Carl Erik Olsen,Meike Burow,Patrick Rollin,Niels Agerbirk +6 more
TL;DR: The diversity of GSLs in plants is critically reviewed here, resulting in significant discrepancies with previous reviews, and the importance of using authentic standards is stressed, including reports based on chromatography hyphenated to MS.
Journal ArticleDOI
JAZ repressors of metabolic defense promote growth and reproductive fitness in Arabidopsis
Qiang Guo,Yuki Yoshida,Ian T. Major,Kun Wang,Koichi Sugimoto,George Kapali,Nathan E. Havko,Christoph Benning,Gregg A. Howe +8 more
TL;DR: Combining mutations within the 13-member Arabidopsis JAZ gene family to investigate the effects of chronic JAZ deficiency on growth, defense, and reproductive output demonstrates that JAZ proteins promote growth and reproductive success at least in part by preventing catastrophic metabolic effects of an unrestrained immune response.
Journal ArticleDOI
An evolutionarily young defense metabolite influences the root growth of plants via the ancient TOR signaling pathway
Frederikke Gro Malinovsky,Marie-Louise F Thomsen,Sebastian J. Nintemann,Lea Møller Jagd,Baptiste Bourgine,Meike Burow,Daniel J. Kliebenstein,Daniel J. Kliebenstein +7 more
TL;DR: This work reports novel signaling properties specific to one distinct glucosinolate, 3-hydroxypropylglucosInolate, which reversibly inhibits root growth and development and raises the possibility that other evolutionarily new plant metabolites may link to ancient signaling pathways.
Journal ArticleDOI
Chemical convergence between plants and insects: biosynthetic origins and functions of common secondary metabolites.
TL;DR: Despite the phylogenetic distance between plants and insects, these two groups of organisms produce some secondary metabolites in common, and researchers have long struggled to find convincing explanations for such co-occurrence.
Journal ArticleDOI
Glutathione Transferase U13 Functions in Pathogen-Triggered Glucosinolate Metabolism
Mariola Piślewska-Bednarek,Ryohei Thomas Nakano,Kei Hiruma,Kei Hiruma,Marta Pastorczyk,Andrea Sánchez-Vallet,Suthitar Singkaravanit-Ogawa,Danuta Ciesiołka,Yoshitaka Takano,Antonio Molina,Paul Schulze-Lefert,Paweł Bednarek +11 more
TL;DR: It is postulated that GSTU13 connects GSH with the pathogen-triggered PEN2 pathway for IG metabolism to deliver metabolites that may have numerous functions in the innate immune system of Arabidopsis.
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