Quantification of Enterovirus RNA in Sludge Samples Using Single Tube Real-Time RT-PCR
S. Monpoeho,A. Dehee,B. Mignotte,L. Schwartzbrod,V. Marechal,J. C. Nicolas,Sylviane Billaudel,Virginie Ferré +7 more
TLDR
A one-step RT-PCR that uses a dual-labeled fluorogenic probe to quantify the 5' noncoding region of enteroviruses and its sensitivity, simplicity and reproducibility render it suitable as a screening method with which to characterize enterovirus RNA.Abstract:
We have developed a quantitative RTPCR method that can be used to determine the amount of enterovirus RNA in urban sludge samples. This method combines Taq-Man® technology with the ABI PrismTM 7700...read more
Citations
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Journal ArticleDOI
Real-time PCR in virology
TL;DR: The background, advantages and limitations of real-time PCR are described, the literature as it applies to virus detection in the routine and research laboratory is reviewed and the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease.
Journal ArticleDOI
Real‐time PCR in the microbiology laboratory
TL;DR: The amplification hardware and the fluorogenic detection chemistries have evolved rapidly and are now more rapid, sensitive and reproducible, while the risk of carryover contamination is minimised.
Journal ArticleDOI
Fecal source tracking, the indicator paradigm, and managing water quality.
TL;DR: A critical review of source tracking with emphasis on the extent to which methods have been tested, when methods are applicable, their shortcomings, and their usefulness in predicting public health risk or pathogen occurrence is presented.
Journal ArticleDOI
Real-time RT-PCR for norovirus screening in shellfish.
TL;DR: Validation of the new real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II confirmed the utility of the genogroup II primer set to detect a large range of different strains circulating in France since 1995, butgenogroup I strains were detected infrequently.
Journal ArticleDOI
Pathogenic Human Viruses in Coastal Waters
TL;DR: This review addresses both historical and recent investigations into viral contamination of marine waters by addressing the lack of sensitive methods to detect the broad range of both bacterial and viral pathogens.
References
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Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
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Gillian Lewis,T G Metcalf +1 more
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