Journal ArticleDOI
Quantitative measurements and modeling of kinetics in nucleic acid monolayer films using SPR spectroscopy
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TLDR
A quantitative study of the kinetics of formation for a two-component tethered ssDNA monolayer film using in situ two-color surface plasmon resonance (SPR) spectroscopy, which can be understood in terms of a simple physical model and self-consistent parameters.Abstract:
We report a quantitative study of the kinetics of formation for a two-component tethered ssDNA monolayer film using in situ two-color surface plasmon resonance (SPR) spectroscopy. The attachment of the DNA to gold is facilitated by functionalization at the 5‘ end with a thiol group connected by a hexamethylene linker (HS-C6-ssDNA). Detailed data analysis is performed by quantitative comparison of the DNA coverage versus time kinetic data obtained from SPRS with numerical solutions for the differential equations for simultaneous adsorption, desorption, and diffusion at the interface. The kinetics of adsorption of HS-C6-ssDNA onto bare gold as well as the kinetics of loss of HS-C6-ssDNA from the surface during subsequent treatment with mercaptohexanol can be understood in terms of a simple physical model and self-consistent parameters. The kinetics of HS-C6-ssDNA adsorption on bare gold are compared to the kinetics of hybridization of surface-attached thiolated ssDNA with the fully complementary ssDNA in fr...read more
Citations
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A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles.
TL;DR: The LSPR nanobiosensor provides a pathway to ultrasensitive biodetection experiments with extremely simple, small, light, robust, low-cost instrumentation that will greatly facilitate field-portable environmental or point-of-service medical diagnostic applications.
Journal ArticleDOI
Electrochemical Biosensors - Sensor Principles and Architectures
TL;DR: In this article, the most common traditional traditional techniques, such as cyclic voltammetry, chronoamperometry, chronopotentiometry, impedance spectroscopy, and various field-effect transistor based methods are presented along with selected promising novel approaches, including nanowire or magnetic nanoparticle-based biosensing.
Journal ArticleDOI
The effect of surface probe density on DNA hybridization
TL;DR: This paper studies how probe immobilization at surfaces, specifically probe density, influences the kinetics of target capture using surface plasmon resonance (SPR) spectroscopy, an in situ label-free optical method.
Journal ArticleDOI
A unified view of propagating and localized surface plasmon resonance biosensors
TL;DR: The color associated with single nanoparticles and surface-confined nanoparticle arrays will be shown to be tunable and useful as platforms for chemical and biological sensing and the LSPR nanosensor will be compared to traditional, flat surface, propagating surface plasmon resonance sensors.
Journal ArticleDOI
Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array.
Rachel McKendry,J. Zhang,Youri Arntz,Torsten Strunz,Martin Hegner,Hans Peter Lang,Marko Baller,Ulrich Certa,Ernst Meyer,Hans-Joachim Güntherodt,Christoph Gerber +10 more
TL;DR: It is shown that cantilever arrays can be used to investigate the thermodynamics of biomolecular interactions mechanically, and it is found that the specificity of the reaction on a cantilevers is consistent with solution data.
References
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Journal ArticleDOI
Characterization of DNA Probes Immobilized on Gold Surfaces
Tonya M. Herne,Michael J. Tarlov +1 more
TL;DR: It is found that hybridization of surface-bound HS-ssDNA is dependent on surface coverage, and nonspecifically adsorbed DNA is largely removed from the surface.
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Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells.
TL;DR: The convenience and broad application offered by SAMs and microcontact printing make this combination of techniques useful for studying a variety of fundamental phenomena in biointerfacial science.
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Molecular interactions on microarrays
TL;DR: This work discusses how arrays of oligonucleotides provide powerful tools to study the molecular basis of these interactions on a scale which is impossible using conventional analysis.
Journal ArticleDOI
Selective reduction of disulfides by tris(2-carboxyethyl) phosphine
TL;DR: Tris(2-carboxyethyl) phosphine (TCEP) is a reagent for disulfide reduction in water at pH 4.5 that preferentially reduces more strained disulfides, in accordance with the usual mechanism postulated for reduction by phosphines in water.