Journal ArticleDOI
Quantitative thin-layer chromatography of glycolipids in animal tissues.
TLDR
The combination of a thin-layer chromatographic system, which makes the previous separation of glycolipids by column chromatography unnecessary, with the orcinol reaction carried out in the presence of silica gel permitted a rapid and simple determination of cerebrosides, sulphatides and monogalactosyl diglyceride with as little as 50 mg of brain tissue.About:
This article is published in Clinica Chimica Acta.The article was published on 1972-04-01. It has received 67 citations till now. The article focuses on the topics: Column chromatography & Thin-layer chromatography.read more
Citations
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Journal ArticleDOI
Lipid analysis by thin-layer chromatography-A review of the current state
TL;DR: The combination betweenHPTLC and mass spectrometric (MS) detection methods will be discussed and it will be shown that this is a very powerful method to investigate the identities of the HPTLC spots in more detail than by the use of common staining methods.
Journal ArticleDOI
The specific interaction of myelin basic protein with lipids at the air-water interface.
TL;DR: The observed specificity, the effects of different solutes, differences in fatty acid chain length of the lipids and the increase in area at constant pressure demonstrate that ionic and hydrophobic forces are involved in the interaction of A1 basic protein and lipids.
Book ChapterDOI
Thin-layer chromatography of neutral glycosphingolipids.
TL;DR: This chapter describes the methods for separation by thin layer chromatography of glycosphingolipids that do not contain sialic acid and are called neutral glycospiringolipid, which include not only genuine neutral glycospherehingolIPids but also sulfatides, psychosines, and ceramides.
Journal ArticleDOI
Microanalysis of brain lipids: multiple two-dimensional thin-layer chromatography.
TL;DR: The techniques described allow the quantitation of cholesterol, cerebrosides, sulfatides, ethanolamine phospholipids, phosphatidylcholine, -serine, -inositol, and gangliosides on 500 micrograms of lipid to study regional differences in the central nervous system, to analyze cell membranes or subcellular particles, or to analyze pathological biopsies in thecentral and peripheral nervous systems.
References
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Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Lipid composition of the normal human brain: gray matter, white matter, and myelin
John S. O'Brien,E. Lois Sampson +1 more
TL;DR: The extra-myelin portion of white matter had a lipid composition that was very similar to that of myelin, but quite different from that of gray matter.
Journal ArticleDOI
Quantitative determination of the neutral glycosyl ceramides in human blood
TL;DR: Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males and varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside.
Journal ArticleDOI
The quantitative estimation of cerebrosides in nervous tissue.
TL;DR: The values for cerebrosides found in the current literature include gangliosides, and other investigators (JOHNSON, MCNABB, and ROSSITER, 1950; CUMINGS, 1953; BLACKWOOD and CUMings, 1954) have neglected the ganglariosides.
Journal ArticleDOI
Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent.
TL;DR: A copper acetate spray reagent has been used for the quantitative densitometric thin-layer chromatography of glycolipids and phospholipids from human CNS myelin and the precision ranges between zero and ± 4.5%.