Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.
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A rapid simple technique based on the sensitive detection of rotavirus double-stranded RNA genome segments separated in polyacrylamide gels, which is comparable with that of electron microscopy or enzyme-linked immunosorbent assay.Abstract:
A rapid simple technique for the diagnosis of rotavirus has been developed based on the sensitive detection of rotavirus double-stranded RNA genome segments separated in polyacrylamide gels. The method utilizes a recently described ultrasensitive silver stain for polypeptides, which can also detect subnanogram amounts of nucleic acid. The sensitivity of the technique is comparable with that of electron microscopy or enzyme-linked immunosorbent assay.read more
Citations
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Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis
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Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic technique.
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Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.
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Rotavirus isolate WI61 representing a presumptive new human serotype.
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References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels
TL;DR: The number of steps in the procedure is reduced from 10 to 6, the reagents in each step are simplified, and the amount of silver required is reduced by a factor of 10, thus greatly reducing the expense of the procedure.
Journal ArticleDOI
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
TL;DR: A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter when employed with two-dimensional electrophoresis.
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Ultrasensitive silver‐based color staining of polypeptides in polyacrylamide gels
TL;DR: The sensitivity of the silver stain process is superior to Coomassie Blue and is comparable to autoradiography after in corporation of conventional levels of 35S‐ methionine.