Journal ArticleDOI
Real-time biomass-concentration monitoring in animal-cell cultures
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TLDR
The recent advances, trends and problems in optical sensors, including the latest laser and infrared probes, are summarized and alternative methods, such as multiple-extinction fluorimetry, real-time imaging and particle-size analysis, are discussed.About:
This article is published in Trends in Biotechnology.The article was published on 1994-08-01. It has received 98 citations till now. The article focuses on the topics: Bioprocess.read more
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Control of fed-batch fermentations.
TL;DR: Advances in control of fed-batch fermentations are reviewed: simple exponential feeding and inferential methods are examined, as are newer methods based on fuzzy control and neural networks that hold promise for optimizing feeds for complex fermentation systems.
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Process analytical technology (PAT) for biopharmaceutical products.
TL;DR: It is the observation that while significant advances have been accomplished with regard to the ability to analyze/monitor key process and quality attributes in the biotech industry, much more needs to be done with regards to utilizing the collected data for subsequent control of the process, to achieve optimum yield and product quality.
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On-line Measurements and Control of Viable Cell Density in Cell Culture Manufacturing Processes using Radio-frequency Impedance
John P. Carvell,Jason E. Dowd +1 more
TL;DR: Radio-frequency (RF) impedance is reviewed as a method for monitoring and controlling cell culture manufacturing processes and is being used to monitor the concentration of live cells immobilized on micro-carriers or packed beds in cGMP processes where traditional off-line live cell counting methods are inaccurate or impossible to perform.
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Perfusion mammalian cell culture for recombinant protein manufacturing - A critical review.
Jean Marc Bielser,Jean Marc Bielser,Moritz Wolf,Jonathan Souquet,Hervé Broly,Massimo Morbidelli +5 more
TL;DR: This review focuses on the application of continuous processing to the first step of the manufacturing process and recent advances in the field that have the potential to support its successful future development are critically discussed.
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Bioprocess monitoring and computer control : Key roots of the current PAT initiative
Beth Junker,Henry Y. Wang +1 more
TL;DR: Key roots of the current PAT initiative in bioprocess monitoring and control are described, focusing on the impact of Danny Wang's research as a professor at MIT, as well as future applications that are likely to encompass additional novel measurement technologies.
References
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Book
Self Organization And Associative Memory
TL;DR: The purpose and nature of Biological Memory, as well as some of the aspects of Memory Aspects, are explained.
Journal ArticleDOI
On-line monitoring of hybridoma cell growth using a laser turbidity sensor.
Konstantin Konstantinov,Rindit Pambayun,Ricaredo M. Matanguihan,Toshiomi Yoshida,Cheryl M. Perusicn,Wei Shou Hu +5 more
TL;DR: Positive results suggest that such laser turbidity sensors will facilitate development of systems for on‐line monitoring and control of animal cell cultivations.
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Fed-batch culture of hybridoma cells: comparison of optimal control approach and closed loop strategies
TL;DR: Control of fed-batch culture of hybridoma cells was investigated based on two approaches optimal control theory and feedback control, finding that there was a delay in the assimilation of the glutamine that should be included in the model to explain the lower MAb production in feedback mode.
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Effects of cell density and glucose and glutamine levels on the respiration rates of hybridoma cells
TL;DR: The experimental oxygen consumption rate was found to be constant over a wide range of dissolved oxygen levels if the suspension medium contained glutamine, however, the rate of oxygen consumption decreased slowly with time in glutamine‐free medium.